Dexamethasone Inhibits White Adipose Tissue Browning

White adipose tissue (WAT) regulates energy balance through energy storage, adipokines secretion and the thermogenesis process. Beige adipocytes are responsible forWAT thermogenesis. They are generated by adipogenesis or transdifferentiation during cold or β3-adrenergic agonist stimulus through a p...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Giordano, Alejandra, Gambaro, Sabrina Eliana, Alzamendi, Ana, Harnichar, Alejandro Ezequiel, Rey, María Amanda, Ongaro, Luisina, Spinedi, Eduardo Julio, Zubiría, María Guillermina, Giovambattista, Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2024
Materias:
Biología
thermogenesis
beige adipocytes
glucocorticoids
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/167351
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-167351
record_format dspace
spelling I19-R120-10915-1673512024-06-18T20:10:32Z http://sedici.unlp.edu.ar/handle/10915/167351 Dexamethasone Inhibits White Adipose Tissue Browning Giordano, Alejandra Gambaro, Sabrina Eliana Alzamendi, Ana Harnichar, Alejandro Ezequiel Rey, María Amanda Ongaro, Luisina Spinedi, Eduardo Julio Zubiría, María Guillermina Giovambattista, Andrés 2024 2024-06-18T16:47:38Z en Biología thermogenesis beige adipocytes glucocorticoids White adipose tissue (WAT) regulates energy balance through energy storage, adipokines secretion and the thermogenesis process. Beige adipocytes are responsible forWAT thermogenesis. They are generated by adipogenesis or transdifferentiation during cold or β3-adrenergic agonist stimulus through a process called browning. Browning has gained significant interest for to its preventive effect on obesity. Glucocorticoids (GCs) have several functions in WAT biology; however, their role in beige adipocyte generation and WAT browning is not fully understood. The aim of our study was to determine the effect of dexamethasone (DXM) on WAT thermogenesis. For this purpose, rats were treated with DXM at room temperature (RT) or cold conditions to determine different thermogenic markers. Furthermore, the effects of DXM on the adipogenic potential of beige precursors and on mature beige adipocytes were evaluated in vitro. Our results showed that DXM decreased UCP-1 mRNA and protein levels, mainly after cold exposure. In vitro studies showed that DXM decreased the expression of a beige precursor marker (Ebf2), affecting their ability to differentiate into beige adipocytes, and inhibited the thermogenic response of mature beige adipocytes (Ucp-1, Dio2 and Pgc1α gene expressions and mitochondrial respiration). Overall, our data strongly suggest that DXM can inhibit the thermogenic program of both retroperitoneal and inguinalWAT depots, an effect that could be exerted, at least partially, by inhibiting de novo cell generation and the thermogenic response in beige adipocytes. Instituto Multidisciplinario de Biología Celular Centro de Endocrinología Experimental y Aplicada Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
thermogenesis
beige adipocytes
glucocorticoids
spellingShingle Biología
thermogenesis
beige adipocytes
glucocorticoids
Giordano, Alejandra
Gambaro, Sabrina Eliana
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Rey, María Amanda
Ongaro, Luisina
Spinedi, Eduardo Julio
Zubiría, María Guillermina
Giovambattista, Andrés
Dexamethasone Inhibits White Adipose Tissue Browning
topic_facet Biología
thermogenesis
beige adipocytes
glucocorticoids
description White adipose tissue (WAT) regulates energy balance through energy storage, adipokines secretion and the thermogenesis process. Beige adipocytes are responsible forWAT thermogenesis. They are generated by adipogenesis or transdifferentiation during cold or β3-adrenergic agonist stimulus through a process called browning. Browning has gained significant interest for to its preventive effect on obesity. Glucocorticoids (GCs) have several functions in WAT biology; however, their role in beige adipocyte generation and WAT browning is not fully understood. The aim of our study was to determine the effect of dexamethasone (DXM) on WAT thermogenesis. For this purpose, rats were treated with DXM at room temperature (RT) or cold conditions to determine different thermogenic markers. Furthermore, the effects of DXM on the adipogenic potential of beige precursors and on mature beige adipocytes were evaluated in vitro. Our results showed that DXM decreased UCP-1 mRNA and protein levels, mainly after cold exposure. In vitro studies showed that DXM decreased the expression of a beige precursor marker (Ebf2), affecting their ability to differentiate into beige adipocytes, and inhibited the thermogenic response of mature beige adipocytes (Ucp-1, Dio2 and Pgc1α gene expressions and mitochondrial respiration). Overall, our data strongly suggest that DXM can inhibit the thermogenic program of both retroperitoneal and inguinalWAT depots, an effect that could be exerted, at least partially, by inhibiting de novo cell generation and the thermogenic response in beige adipocytes.
format Articulo
Articulo
author Giordano, Alejandra
Gambaro, Sabrina Eliana
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Rey, María Amanda
Ongaro, Luisina
Spinedi, Eduardo Julio
Zubiría, María Guillermina
Giovambattista, Andrés
author_facet Giordano, Alejandra
Gambaro, Sabrina Eliana
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Rey, María Amanda
Ongaro, Luisina
Spinedi, Eduardo Julio
Zubiría, María Guillermina
Giovambattista, Andrés
author_sort Giordano, Alejandra
title Dexamethasone Inhibits White Adipose Tissue Browning
title_short Dexamethasone Inhibits White Adipose Tissue Browning
title_full Dexamethasone Inhibits White Adipose Tissue Browning
title_fullStr Dexamethasone Inhibits White Adipose Tissue Browning
title_full_unstemmed Dexamethasone Inhibits White Adipose Tissue Browning
title_sort dexamethasone inhibits white adipose tissue browning
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/167351
work_keys_str_mv AT giordanoalejandra dexamethasoneinhibitswhiteadiposetissuebrowning
AT gambarosabrinaeliana dexamethasoneinhibitswhiteadiposetissuebrowning
AT alzamendiana dexamethasoneinhibitswhiteadiposetissuebrowning
AT harnicharalejandroezequiel dexamethasoneinhibitswhiteadiposetissuebrowning
AT reymariaamanda dexamethasoneinhibitswhiteadiposetissuebrowning
AT ongaroluisina dexamethasoneinhibitswhiteadiposetissuebrowning
AT spinedieduardojulio dexamethasoneinhibitswhiteadiposetissuebrowning
AT zubiriamariaguillermina dexamethasoneinhibitswhiteadiposetissuebrowning
AT giovambattistaandres dexamethasoneinhibitswhiteadiposetissuebrowning
_version_ 1807223487048187904

Ejemplares similares