Biomodification of acenocoumarol by bifidobacteria

The increased interest of consumers in probiotic foods requires a deeper knowledge on the possible interactions with drugs, because their pharmacological properties could be modified. In this context, these studies are relevant for drugs such as acenocoumarol, whose dosage must be controlled due to,...

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Autores principales: Fragomeno, Melisa, Assad, Sabrina, Mobili, Pablo, Peruzzo, Pablo José, Minnaard, Jessica, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Química
bifidobacteria
biomodification
enzymes
acenocoumarol
probiotic
anticoagulants
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/167134
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-167134
record_format dspace
spelling I19-R120-10915-1671342024-06-12T20:09:34Z http://sedici.unlp.edu.ar/handle/10915/167134 Biomodification of acenocoumarol by bifidobacteria Fragomeno, Melisa Assad, Sabrina Mobili, Pablo Peruzzo, Pablo José Minnaard, Jessica Pérez, Pablo Fernando 2021 2024-06-12T15:08:09Z en Química bifidobacteria biomodification enzymes acenocoumarol probiotic anticoagulants The increased interest of consumers in probiotic foods requires a deeper knowledge on the possible interactions with drugs, because their pharmacological properties could be modified. In this context, these studies are relevant for drugs such as acenocoumarol, whose dosage must be controlled due to, among other factors, food-drug interactions. Acenocoumarol is an oral anticoagulant with a narrow therapeutic range. The aim of the present research is to evaluate, in vitro, the effect of bifidobacteria on acenocoumarol. The drug was incubated with Bifidobacterium bifidum CIDCA 5310 or Bifidobacterium adolescentis CIDCA 5317 in MRS broth at 37◦C for 24 h in anaerobic conditions. The effect of incubation with sterilized spent culture supernatants (SSCS) was also evaluated. Analysis by RP-HPLC showed that both bifidobacterial strains reduced the area of the acenocoumarol peak and two new peaks were evidenced. In addition, a decrease in the intensity of the bands at 1650, 1390 and 1110/cm was observed in the FTIR spectroscopic determinations. Moreover, a new band appeared at 1720/cm. No effect on the drug was observed when incubation was performed with SSCS. The present study showed a significant change in the concentration of the anticoagulant after incubation with bifidobacteria and results are compatible with biomodification of the drug due to enzymatic activity of bifidobacteria. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
bifidobacteria
biomodification
enzymes
acenocoumarol
probiotic
anticoagulants
spellingShingle Química
bifidobacteria
biomodification
enzymes
acenocoumarol
probiotic
anticoagulants
Fragomeno, Melisa
Assad, Sabrina
Mobili, Pablo
Peruzzo, Pablo José
Minnaard, Jessica
Pérez, Pablo Fernando
Biomodification of acenocoumarol by bifidobacteria
topic_facet Química
bifidobacteria
biomodification
enzymes
acenocoumarol
probiotic
anticoagulants
description The increased interest of consumers in probiotic foods requires a deeper knowledge on the possible interactions with drugs, because their pharmacological properties could be modified. In this context, these studies are relevant for drugs such as acenocoumarol, whose dosage must be controlled due to, among other factors, food-drug interactions. Acenocoumarol is an oral anticoagulant with a narrow therapeutic range. The aim of the present research is to evaluate, in vitro, the effect of bifidobacteria on acenocoumarol. The drug was incubated with Bifidobacterium bifidum CIDCA 5310 or Bifidobacterium adolescentis CIDCA 5317 in MRS broth at 37◦C for 24 h in anaerobic conditions. The effect of incubation with sterilized spent culture supernatants (SSCS) was also evaluated. Analysis by RP-HPLC showed that both bifidobacterial strains reduced the area of the acenocoumarol peak and two new peaks were evidenced. In addition, a decrease in the intensity of the bands at 1650, 1390 and 1110/cm was observed in the FTIR spectroscopic determinations. Moreover, a new band appeared at 1720/cm. No effect on the drug was observed when incubation was performed with SSCS. The present study showed a significant change in the concentration of the anticoagulant after incubation with bifidobacteria and results are compatible with biomodification of the drug due to enzymatic activity of bifidobacteria.
format Articulo
Articulo
author Fragomeno, Melisa
Assad, Sabrina
Mobili, Pablo
Peruzzo, Pablo José
Minnaard, Jessica
Pérez, Pablo Fernando
author_facet Fragomeno, Melisa
Assad, Sabrina
Mobili, Pablo
Peruzzo, Pablo José
Minnaard, Jessica
Pérez, Pablo Fernando
author_sort Fragomeno, Melisa
title Biomodification of acenocoumarol by bifidobacteria
title_short Biomodification of acenocoumarol by bifidobacteria
title_full Biomodification of acenocoumarol by bifidobacteria
title_fullStr Biomodification of acenocoumarol by bifidobacteria
title_full_unstemmed Biomodification of acenocoumarol by bifidobacteria
title_sort biomodification of acenocoumarol by bifidobacteria
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/167134
work_keys_str_mv AT fragomenomelisa biomodificationofacenocoumarolbybifidobacteria
AT assadsabrina biomodificationofacenocoumarolbybifidobacteria
AT mobilipablo biomodificationofacenocoumarolbybifidobacteria
AT peruzzopablojose biomodificationofacenocoumarolbybifidobacteria
AT minnaardjessica biomodificationofacenocoumarolbybifidobacteria
AT perezpablofernando biomodificationofacenocoumarolbybifidobacteria
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