Murine model of Bacillus cereus gastrointestinal infection

Bacillus cereus is a spore-forming micro-organism responsible for foodborne illness. In this study, we focus on the host response following intragastric challenge with a pathogenic B. cereus strain (B10502) isolated from a foodborne outbreak. C57BL/6J female mice were infected by gavage with strain...

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Autores principales: Rolny, Ivanna Sabrina, Minnaard, Jessica, Racedo, Silvia María, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Química
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/167133
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-167133
record_format dspace
spelling I19-R120-10915-1671332024-06-12T20:09:37Z http://sedici.unlp.edu.ar/handle/10915/167133 Murine model of Bacillus cereus gastrointestinal infection Rolny, Ivanna Sabrina Minnaard, Jessica Racedo, Silvia María Pérez, Pablo Fernando 2014 2024-06-12T15:00:17Z en Química Bacillus cereus is a spore-forming micro-organism responsible for foodborne illness. In this study, we focus on the host response following intragastric challenge with a pathogenic B. cereus strain (B10502) isolated from a foodborne outbreak. C57BL/6J female mice were infected by gavage with strain B10502. Controls were administered with PBS. Infection leads to significant modification in relevant immune cells in the spleen, Peyer’s patches (PP) and mesenteric lymph nodes (MLN). These findings correlated with an increase in the size of PP as compared with uninfected controls. Histological studies showed that B. cereus infection increased the ratio of intestinal goblet cells and induces mononuclear cell infiltrates in spleen at 5 days post-infection. Evaluation of cytokine mRNA expression demonstrated a significant increase in IFN-c in MLN after 2 days of infection. The present work demonstrates that infection of mice with vegetative B. cereus is self-limited. Our findings determined relevant cell populations that were involved in the control of the pathogen through modification of the ratio and/or activation. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 1741-1749
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
spellingShingle Química
Rolny, Ivanna Sabrina
Minnaard, Jessica
Racedo, Silvia María
Pérez, Pablo Fernando
Murine model of Bacillus cereus gastrointestinal infection
topic_facet Química
description Bacillus cereus is a spore-forming micro-organism responsible for foodborne illness. In this study, we focus on the host response following intragastric challenge with a pathogenic B. cereus strain (B10502) isolated from a foodborne outbreak. C57BL/6J female mice were infected by gavage with strain B10502. Controls were administered with PBS. Infection leads to significant modification in relevant immune cells in the spleen, Peyer’s patches (PP) and mesenteric lymph nodes (MLN). These findings correlated with an increase in the size of PP as compared with uninfected controls. Histological studies showed that B. cereus infection increased the ratio of intestinal goblet cells and induces mononuclear cell infiltrates in spleen at 5 days post-infection. Evaluation of cytokine mRNA expression demonstrated a significant increase in IFN-c in MLN after 2 days of infection. The present work demonstrates that infection of mice with vegetative B. cereus is self-limited. Our findings determined relevant cell populations that were involved in the control of the pathogen through modification of the ratio and/or activation.
format Articulo
Articulo
author Rolny, Ivanna Sabrina
Minnaard, Jessica
Racedo, Silvia María
Pérez, Pablo Fernando
author_facet Rolny, Ivanna Sabrina
Minnaard, Jessica
Racedo, Silvia María
Pérez, Pablo Fernando
author_sort Rolny, Ivanna Sabrina
title Murine model of Bacillus cereus gastrointestinal infection
title_short Murine model of Bacillus cereus gastrointestinal infection
title_full Murine model of Bacillus cereus gastrointestinal infection
title_fullStr Murine model of Bacillus cereus gastrointestinal infection
title_full_unstemmed Murine model of Bacillus cereus gastrointestinal infection
title_sort murine model of bacillus cereus gastrointestinal infection
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/167133
work_keys_str_mv AT rolnyivannasabrina murinemodelofbacilluscereusgastrointestinalinfection
AT minnaardjessica murinemodelofbacilluscereusgastrointestinalinfection
AT racedosilviamaria murinemodelofbacilluscereusgastrointestinalinfection
AT perezpablofernando murinemodelofbacilluscereusgastrointestinalinfection
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