Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage

1–Methylcyclopropene (1–MCP) is used for extending the postharvest life of the avocado during storage. In this study, fruits from two maturity stages at harvest were used: low dry matter (20–23%) and high dry matter (27%), combined with 1–MCP at different times after harvest, i.e., 0, 7, 14, and 21...

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Detalles Bibliográficos
Autores principales: Olivares, Daniela, García-Rojas, Miguel, Ulloa, Pablo, Riveros, Aníbal, Pedreschi, Romina, Campos-Vargas, Reinaldo, Meneses, Claudiio, Defilippi, Bruno
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Ciencias Agrarias
Ethylene
1-MCP
RNA-seq, quality
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/166943
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-166943
record_format dspace
spelling I19-R120-10915-1669432024-07-02T17:21:51Z http://sedici.unlp.edu.ar/handle/10915/166943 Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage Olivares, Daniela García-Rojas, Miguel Ulloa, Pablo Riveros, Aníbal Pedreschi, Romina Campos-Vargas, Reinaldo Meneses, Claudiio Defilippi, Bruno 2023-12 2024-06-07T17:22:01Z en Ciencias Agrarias Ethylene 1-MCP RNA-seq, quality 1–Methylcyclopropene (1–MCP) is used for extending the postharvest life of the avocado during storage. In this study, fruits from two maturity stages at harvest were used: low dry matter (20–23%) and high dry matter (27%), combined with 1–MCP at different times after harvest, i.e., 0, 7, 14, and 21 d at 5 °C, to identify the threshold of the ethylene inhibition response in “Hass” avocado. Our results showed changes in ethylene production rates and transcript accumulation of genes involved in ethylene metabolism when measured at harvest and during storage. 1–MCP treated fruit up to 14 d of storage showed similar values of firmness and skin color as fruit treated at harvest time. In contrast, when the application was performed after 21 d, the fruit showed ripening attributes like those of the untreated ones. We performed a transcriptomic analysis, to further understand the lack of response to 1-MCP at 21 d of storage. Results showed an increase of transcripts involved in the ‘response to ethylene’. All genes evaluated showed similar expression profiles induced by cold storage time, with a peak at 21 d of storage and an increased softening of the fruit and peel color. Our results should help to understand the molecular mechanisms of the avocado ripening process. Facultad de Ciencias Agrarias y Forestales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 97-97
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ethylene
1-MCP
RNA-seq, quality
spellingShingle Ciencias Agrarias
Ethylene
1-MCP
RNA-seq, quality
Olivares, Daniela
García-Rojas, Miguel
Ulloa, Pablo
Riveros, Aníbal
Pedreschi, Romina
Campos-Vargas, Reinaldo
Meneses, Claudiio
Defilippi, Bruno
Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
topic_facet Ciencias Agrarias
Ethylene
1-MCP
RNA-seq, quality
description 1–Methylcyclopropene (1–MCP) is used for extending the postharvest life of the avocado during storage. In this study, fruits from two maturity stages at harvest were used: low dry matter (20–23%) and high dry matter (27%), combined with 1–MCP at different times after harvest, i.e., 0, 7, 14, and 21 d at 5 °C, to identify the threshold of the ethylene inhibition response in “Hass” avocado. Our results showed changes in ethylene production rates and transcript accumulation of genes involved in ethylene metabolism when measured at harvest and during storage. 1–MCP treated fruit up to 14 d of storage showed similar values of firmness and skin color as fruit treated at harvest time. In contrast, when the application was performed after 21 d, the fruit showed ripening attributes like those of the untreated ones. We performed a transcriptomic analysis, to further understand the lack of response to 1-MCP at 21 d of storage. Results showed an increase of transcripts involved in the ‘response to ethylene’. All genes evaluated showed similar expression profiles induced by cold storage time, with a peak at 21 d of storage and an increased softening of the fruit and peel color. Our results should help to understand the molecular mechanisms of the avocado ripening process.
format Articulo
Articulo
author Olivares, Daniela
García-Rojas, Miguel
Ulloa, Pablo
Riveros, Aníbal
Pedreschi, Romina
Campos-Vargas, Reinaldo
Meneses, Claudiio
Defilippi, Bruno
author_facet Olivares, Daniela
García-Rojas, Miguel
Ulloa, Pablo
Riveros, Aníbal
Pedreschi, Romina
Campos-Vargas, Reinaldo
Meneses, Claudiio
Defilippi, Bruno
author_sort Olivares, Daniela
title Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
title_short Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
title_full Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
title_fullStr Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
title_full_unstemmed Response mechanisms of “Hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
title_sort response mechanisms of “hass” avocado to sequential 1–methylcyclopropene applications at different maturity stages during cold storage
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/166943
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