Bifidobacteria from human origin: interaction with phagocytic cells

Aim of the study: Given that phagocytic cells are main players of the host immune response, we studied the interaction of bifidobacteria with monocytic THP-1 cells in nonopsonic conditions. Methods and Results: Association/internalization, cell response (expression of HLA-DR and TLR2), M1/M2 macrop...

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Autores principales: Assad, Sabrina, Rolny, Ivanna Sabrina, Minnaard, Jessica, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Química
fermented foods
immunology
intestinal microbiology
mechanism of action
probiotics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/166689
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-166689
record_format dspace
spelling I19-R120-10915-1666892024-06-03T20:08:08Z http://sedici.unlp.edu.ar/handle/10915/166689 Bifidobacteria from human origin: interaction with phagocytic cells Assad, Sabrina Rolny, Ivanna Sabrina Minnaard, Jessica Pérez, Pablo Fernando 2020 2024-06-03T18:44:52Z en Química fermented foods immunology intestinal microbiology mechanism of action probiotics Aim of the study: Given that phagocytic cells are main players of the host immune response, we studied the interaction of bifidobacteria with monocytic THP-1 cells in nonopsonic conditions. Methods and Results: Association/internalization, cell response (expression of HLA-DR and TLR2), M1/M2 macrophage polarization and colocalization of micro-organisms with Lysotracker or transferrin were evaluated. Screening with eight Bifidobacterium strains showed two patterns of interactions with THP-1 cells: high and low association and phagocytosis. Two strains with different surface properties were further studied: B. bifidum CIDCA 5310 and B. adolescentis CIDCA 5317. Strain CIDCA 5310 showed higher levels of colocalization in lysosome than strain CIDCA 5317. Both strains stimulated TLR2 expression. Strain CIDCA 5317 significantly increases HLA-DR expression, however, when cells are stimulated with IFN-c, strain CIDCA 5310 induces the highest value of expression. Noteworthy, strain CIDCA 5310 was able to upregulate both M1 and M2 markers of macrophage polarization. Conclusions: Our results demonstrate that bifidobacteria from human origin show different patterns of interaction with phagocytic cells thus leading to different cell responses. These findings add further insight on the mechanisms involved in the biologic effects of probiotics. Significance and Impact of the Study: Knowledge of the interaction of bifidobacteria with key players of the host immune response is paramount for the understanding of the mechanisms involved in the beneficial effects. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Instituto de Estudios Inmunológicos y Fisiopatológicos Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
fermented foods
immunology
intestinal microbiology
mechanism of action
probiotics
spellingShingle Química
fermented foods
immunology
intestinal microbiology
mechanism of action
probiotics
Assad, Sabrina
Rolny, Ivanna Sabrina
Minnaard, Jessica
Pérez, Pablo Fernando
Bifidobacteria from human origin: interaction with phagocytic cells
topic_facet Química
fermented foods
immunology
intestinal microbiology
mechanism of action
probiotics
description Aim of the study: Given that phagocytic cells are main players of the host immune response, we studied the interaction of bifidobacteria with monocytic THP-1 cells in nonopsonic conditions. Methods and Results: Association/internalization, cell response (expression of HLA-DR and TLR2), M1/M2 macrophage polarization and colocalization of micro-organisms with Lysotracker or transferrin were evaluated. Screening with eight Bifidobacterium strains showed two patterns of interactions with THP-1 cells: high and low association and phagocytosis. Two strains with different surface properties were further studied: B. bifidum CIDCA 5310 and B. adolescentis CIDCA 5317. Strain CIDCA 5310 showed higher levels of colocalization in lysosome than strain CIDCA 5317. Both strains stimulated TLR2 expression. Strain CIDCA 5317 significantly increases HLA-DR expression, however, when cells are stimulated with IFN-c, strain CIDCA 5310 induces the highest value of expression. Noteworthy, strain CIDCA 5310 was able to upregulate both M1 and M2 markers of macrophage polarization. Conclusions: Our results demonstrate that bifidobacteria from human origin show different patterns of interaction with phagocytic cells thus leading to different cell responses. These findings add further insight on the mechanisms involved in the biologic effects of probiotics. Significance and Impact of the Study: Knowledge of the interaction of bifidobacteria with key players of the host immune response is paramount for the understanding of the mechanisms involved in the beneficial effects.
format Articulo
Articulo
author Assad, Sabrina
Rolny, Ivanna Sabrina
Minnaard, Jessica
Pérez, Pablo Fernando
author_facet Assad, Sabrina
Rolny, Ivanna Sabrina
Minnaard, Jessica
Pérez, Pablo Fernando
author_sort Assad, Sabrina
title Bifidobacteria from human origin: interaction with phagocytic cells
title_short Bifidobacteria from human origin: interaction with phagocytic cells
title_full Bifidobacteria from human origin: interaction with phagocytic cells
title_fullStr Bifidobacteria from human origin: interaction with phagocytic cells
title_full_unstemmed Bifidobacteria from human origin: interaction with phagocytic cells
title_sort bifidobacteria from human origin: interaction with phagocytic cells
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/166689
work_keys_str_mv AT assadsabrina bifidobacteriafromhumanorigininteractionwithphagocyticcells
AT rolnyivannasabrina bifidobacteriafromhumanorigininteractionwithphagocyticcells
AT minnaardjessica bifidobacteriafromhumanorigininteractionwithphagocyticcells
AT perezpablofernando bifidobacteriafromhumanorigininteractionwithphagocyticcells
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