The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol

Bifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. B...

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Autores principales: Assad, Sabrina, Fragomeno, Melisa, Rumbo, Martín, Minnaard, Jessica, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/166688
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-166688
record_format dspace
spelling I19-R120-10915-1666882024-06-03T20:08:13Z http://sedici.unlp.edu.ar/handle/10915/166688 The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol Assad, Sabrina Fragomeno, Melisa Rumbo, Martín Minnaard, Jessica Pérez, Pablo Fernando 2023 2024-06-03T18:34:00Z en Química Bifidobacterium Acenocoumarol THP-1 cells Caco-2 ccl20:luc reporter system Immune response Anticoagulant Bifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. Bifidobacterium bifidum CIDCA 5310 and B. adolescentis CIDCA 5317 were grown in MRS broth in anaerobic conditions. The experiments were done in the presence of acenocoumarol. FITC-labeled bacteria were incubated with THP1 cells differentiated with PMA (THP1-PMA), and phagocytosis and intracellular localization of bacteria were assessed by flow cytometry and confocal laser microscopy, respectively. Also, expression of macrophage surface molecules in THP1 and THP1-PMA cells was assessed by flow cytometry. By using the Caco-2 ccl20:luc reporter system innate immune response was analysed by determining luciferase expression induced by flagellin. Acenocoumarol decreased phagocytosis of bifidobacteria but intracellular routing was not modified. Also, anticoagulant reduced the expression of HLA-DR and CD64 induced by bifidobacteria in both THP1-PMA and THP1 cells. Instead, the decrease in the expression of CD206 and CD23 was dependent on differentiation status of THP1 cells. Acenocoumarol increased CCL20 expression and it was partially abrogated by strain CIDCA 5310. Presence of acenocoumarol modifies the interaction between bifidobacteria and eukaryotic cells thus suggesting that the expected modulation of immune response by probiotic foods can be different in persons under anticoagulant treatment. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Instituto de Estudios Inmunológicos y Fisiopatológicos Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
spellingShingle Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
Assad, Sabrina
Fragomeno, Melisa
Rumbo, Martín
Minnaard, Jessica
Pérez, Pablo Fernando
The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
topic_facet Química
Bifidobacterium
Acenocoumarol
THP-1 cells
Caco-2 ccl20:luc reporter system
Immune response
Anticoagulant
description Bifidobacteria are health promoting microorganisms that can interact with drugs in the intestinal tract thus modifying the activation of immune cells and host homeostasis. To study the effect of acenocoumarol on the interaction between bifidobacteria and THP-1 cells or intestinal epithelial cells. Bifidobacterium bifidum CIDCA 5310 and B. adolescentis CIDCA 5317 were grown in MRS broth in anaerobic conditions. The experiments were done in the presence of acenocoumarol. FITC-labeled bacteria were incubated with THP1 cells differentiated with PMA (THP1-PMA), and phagocytosis and intracellular localization of bacteria were assessed by flow cytometry and confocal laser microscopy, respectively. Also, expression of macrophage surface molecules in THP1 and THP1-PMA cells was assessed by flow cytometry. By using the Caco-2 ccl20:luc reporter system innate immune response was analysed by determining luciferase expression induced by flagellin. Acenocoumarol decreased phagocytosis of bifidobacteria but intracellular routing was not modified. Also, anticoagulant reduced the expression of HLA-DR and CD64 induced by bifidobacteria in both THP1-PMA and THP1 cells. Instead, the decrease in the expression of CD206 and CD23 was dependent on differentiation status of THP1 cells. Acenocoumarol increased CCL20 expression and it was partially abrogated by strain CIDCA 5310. Presence of acenocoumarol modifies the interaction between bifidobacteria and eukaryotic cells thus suggesting that the expected modulation of immune response by probiotic foods can be different in persons under anticoagulant treatment.
format Articulo
Articulo
author Assad, Sabrina
Fragomeno, Melisa
Rumbo, Martín
Minnaard, Jessica
Pérez, Pablo Fernando
author_facet Assad, Sabrina
Fragomeno, Melisa
Rumbo, Martín
Minnaard, Jessica
Pérez, Pablo Fernando
author_sort Assad, Sabrina
title The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_short The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_full The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_fullStr The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_full_unstemmed The immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
title_sort immunomodulating effect of bifidobacteria is modified by the anticoagulant acenocoumarol
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/166688
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