Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum

Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT), initially restricted to South America, is a global threat for wheat after spreading to Asia in 2016 by the introduction of contaminated seeds, raising the question about transmission of the pathogen from seeds to seedlings, a process...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Martínez, Sergio Iván, Wegner, Alex, Bohnert, Stefan, Schaffrath, Ulrich, Perelló, Analía Edith
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Ciencias Agrarias
Magnaporthe oryzae
reisolation
seed transmission
seedborne disease
wheat blast
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/164084
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-164084
record_format dspace
spelling I19-R120-10915-1640842024-03-20T20:06:39Z http://sedici.unlp.edu.ar/handle/10915/164084 Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum Martínez, Sergio Iván Wegner, Alex Bohnert, Stefan Schaffrath, Ulrich Perelló, Analía Edith 2021 2024-03-20T18:56:18Z en Ciencias Agrarias Magnaporthe oryzae reisolation seed transmission seedborne disease wheat blast Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT), initially restricted to South America, is a global threat for wheat after spreading to Asia in 2016 by the introduction of contaminated seeds, raising the question about transmission of the pathogen from seeds to seedlings, a process so far not well understood. We therefore studied the relationship between seed infection and disease symptoms on seedlings and adult plants. To accomplish this objective, we inoculated spikes of wheat cv. Apogee with a transgenic isolate (MoT-DsRed, with the addition of being resistant to hygromycin). We identified MoT-DsRed in experiments using hygromycin resistance for selection or by observation of DsRed fluorescence. The seeds from infected plants looked either apparently healthy or shrivelled. To evaluate the transmission, two experimental designs were chosen (blotter test and greenhouse) and MoT-DsRed was recovered from both. This revealed that MoT is able to colonize wheat seedlings from infected seeds under the ground. The favourable conditions of temperature and humidity allowed a high recovery rate of MoT from wheat shoots when grown in artificial media. Around 42 days after germination of infected seeds, MoT-DsRed could not be reisolated, indicating that fungal progression, at this time point, did not proceed systemically/endophytically. We hypothesize that spike infection might occur via spore dispersal from infected leaves rather than within the plant. Because MoT-DsRed was not only successfully reisolated from seed coats and germinating seeds with symptoms, but also from apparently healthy seeds, urgent attention is needed to minimize the risks of inadvertent dispersal of inoculum. Facultad de Ciencias Agrarias y Forestales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 1562-1571
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Magnaporthe oryzae
reisolation
seed transmission
seedborne disease
wheat blast
spellingShingle Ciencias Agrarias
Magnaporthe oryzae
reisolation
seed transmission
seedborne disease
wheat blast
Martínez, Sergio Iván
Wegner, Alex
Bohnert, Stefan
Schaffrath, Ulrich
Perelló, Analía Edith
Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum
topic_facet Ciencias Agrarias
Magnaporthe oryzae
reisolation
seed transmission
seedborne disease
wheat blast
description Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT), initially restricted to South America, is a global threat for wheat after spreading to Asia in 2016 by the introduction of contaminated seeds, raising the question about transmission of the pathogen from seeds to seedlings, a process so far not well understood. We therefore studied the relationship between seed infection and disease symptoms on seedlings and adult plants. To accomplish this objective, we inoculated spikes of wheat cv. Apogee with a transgenic isolate (MoT-DsRed, with the addition of being resistant to hygromycin). We identified MoT-DsRed in experiments using hygromycin resistance for selection or by observation of DsRed fluorescence. The seeds from infected plants looked either apparently healthy or shrivelled. To evaluate the transmission, two experimental designs were chosen (blotter test and greenhouse) and MoT-DsRed was recovered from both. This revealed that MoT is able to colonize wheat seedlings from infected seeds under the ground. The favourable conditions of temperature and humidity allowed a high recovery rate of MoT from wheat shoots when grown in artificial media. Around 42 days after germination of infected seeds, MoT-DsRed could not be reisolated, indicating that fungal progression, at this time point, did not proceed systemically/endophytically. We hypothesize that spike infection might occur via spore dispersal from infected leaves rather than within the plant. Because MoT-DsRed was not only successfully reisolated from seed coats and germinating seeds with symptoms, but also from apparently healthy seeds, urgent attention is needed to minimize the risks of inadvertent dispersal of inoculum.
format Articulo
Articulo
author Martínez, Sergio Iván
Wegner, Alex
Bohnert, Stefan
Schaffrath, Ulrich
Perelló, Analía Edith
author_facet Martínez, Sergio Iván
Wegner, Alex
Bohnert, Stefan
Schaffrath, Ulrich
Perelló, Analía Edith
author_sort Martínez, Sergio Iván
title Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum
title_short Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum
title_full Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum
title_fullStr Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum
title_full_unstemmed Tracing seed to seedling transmission of the wheat blast pathogen Magnaporthe oryzae pathotype Triticum
title_sort tracing seed to seedling transmission of the wheat blast pathogen magnaporthe oryzae pathotype triticum
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/164084
work_keys_str_mv AT martinezsergioivan tracingseedtoseedlingtransmissionofthewheatblastpathogenmagnaportheoryzaepathotypetriticum
AT wegneralex tracingseedtoseedlingtransmissionofthewheatblastpathogenmagnaportheoryzaepathotypetriticum
AT bohnertstefan tracingseedtoseedlingtransmissionofthewheatblastpathogenmagnaportheoryzaepathotypetriticum
AT schaffrathulrich tracingseedtoseedlingtransmissionofthewheatblastpathogenmagnaportheoryzaepathotypetriticum
AT perelloanaliaedith tracingseedtoseedlingtransmissionofthewheatblastpathogenmagnaportheoryzaepathotypetriticum
_version_ 1807222722382528512

Ejemplares similares