Hypophysotropic activity of histone H3 in vitro

To assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the rele...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Brown, Oscar Alfredo, Sosa, Yolanda Elena, Naumovich, Diana, Hereñú, Claudia Beatriz, Goya, Rodolfo Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/164076
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-164076
record_format dspace
spelling I19-R120-10915-1640762024-03-21T04:07:50Z http://sedici.unlp.edu.ar/handle/10915/164076 Hypophysotropic activity of histone H3 in vitro Brown, Oscar Alfredo Sosa, Yolanda Elena Naumovich, Diana Hereñú, Claudia Beatriz Goya, Rodolfo Gustavo 2003 2024-03-20T18:03:17Z en Bioquímica Histones Hypophysotropic activity Pituitary cells Second messengers Apoptotic signals To assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6mMH3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals. Instituto de Investigaciones Bioquímicas de La Plata Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 671-678
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
spellingShingle Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
Brown, Oscar Alfredo
Sosa, Yolanda Elena
Naumovich, Diana
Hereñú, Claudia Beatriz
Goya, Rodolfo Gustavo
Hypophysotropic activity of histone H3 in vitro
topic_facet Bioquímica
Histones
Hypophysotropic activity
Pituitary cells
Second messengers
Apoptotic signals
description To assess the effect of histoneH3on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30mM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6mMH3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals.
format Articulo
Articulo
author Brown, Oscar Alfredo
Sosa, Yolanda Elena
Naumovich, Diana
Hereñú, Claudia Beatriz
Goya, Rodolfo Gustavo
author_facet Brown, Oscar Alfredo
Sosa, Yolanda Elena
Naumovich, Diana
Hereñú, Claudia Beatriz
Goya, Rodolfo Gustavo
author_sort Brown, Oscar Alfredo
title Hypophysotropic activity of histone H3 in vitro
title_short Hypophysotropic activity of histone H3 in vitro
title_full Hypophysotropic activity of histone H3 in vitro
title_fullStr Hypophysotropic activity of histone H3 in vitro
title_full_unstemmed Hypophysotropic activity of histone H3 in vitro
title_sort hypophysotropic activity of histone h3 in vitro
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/164076
work_keys_str_mv AT brownoscaralfredo hypophysotropicactivityofhistoneh3invitro
AT sosayolandaelena hypophysotropicactivityofhistoneh3invitro
AT naumovichdiana hypophysotropicactivityofhistoneh3invitro
AT herenuclaudiabeatriz hypophysotropicactivityofhistoneh3invitro
AT goyarodolfogustavo hypophysotropicactivityofhistoneh3invitro
_version_ 1807222720460488704

Ejemplares similares