Terpolymer-chitosan membranes as biomaterial

The present study shows a novel copolymer synthesis, its application in the membrane design and the physicochemical and biological characterization of the biomaterial obtained. Terpolymer starting diisopropyl fumarate (F), vinyl benzoate (V) and 2-hydroxyethyl methacrylate (H) was prepared by therma...

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Autores principales: Bravi Costantino, Maria Leticia, Belluzo, María Soledad, Oberti, Tamara Gisela, Cortizo, Ana María, Cortizo, María Susana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Química
Biología
biomaterials
chitosan
cytotoxicity
fumaric polymers
swelling
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/164070
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-164070
record_format dspace
spelling I19-R120-10915-1640702024-03-21T04:07:11Z http://sedici.unlp.edu.ar/handle/10915/164070 Terpolymer-chitosan membranes as biomaterial Bravi Costantino, Maria Leticia Belluzo, María Soledad Oberti, Tamara Gisela Cortizo, Ana María Cortizo, María Susana 2022-02 2024-03-20T17:08:16Z en Química Biología biomaterials chitosan cytotoxicity fumaric polymers swelling The present study shows a novel copolymer synthesis, its application in the membrane design and the physicochemical and biological characterization of the biomaterial obtained. Terpolymer starting diisopropyl fumarate (F), vinyl benzoate (V) and 2-hydroxyethyl methacrylate (H) was prepared by thermal radical polymerization. This polymer (FVH) was obtained in several monomer ratios and characterized by spectroscopic and chromatographic methods (FTIR, ¹H-NMR and SEC). The best relationship of F:V:H was 5:4:1, which allows efficient interaction with chitosan through cross-linking with borax to achieve scaffolds for potential biomedical applications. The membranes were obtained by solvent casting and analyzed by scanning electron microscopy (SEM), swelling behavior and mechanical properties. In addition, we studied the possible cytotoxicity and biocompatibility of these materials using a murine macrophage-like cell line (RAW 264.7) and bone marrow mesenchymal progenitor cells (BMPC), respectively, taking into account their intended applications. The results of this study show that the terpolymer obtained and its combination with a natural polymer is a very interesting strategy to obtain a biomaterial with possible applications in regenerative medicine and this could be extended to other structurally related systems. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 383-393
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biología
biomaterials
chitosan
cytotoxicity
fumaric polymers
swelling
spellingShingle Química
Biología
biomaterials
chitosan
cytotoxicity
fumaric polymers
swelling
Bravi Costantino, Maria Leticia
Belluzo, María Soledad
Oberti, Tamara Gisela
Cortizo, Ana María
Cortizo, María Susana
Terpolymer-chitosan membranes as biomaterial
topic_facet Química
Biología
biomaterials
chitosan
cytotoxicity
fumaric polymers
swelling
description The present study shows a novel copolymer synthesis, its application in the membrane design and the physicochemical and biological characterization of the biomaterial obtained. Terpolymer starting diisopropyl fumarate (F), vinyl benzoate (V) and 2-hydroxyethyl methacrylate (H) was prepared by thermal radical polymerization. This polymer (FVH) was obtained in several monomer ratios and characterized by spectroscopic and chromatographic methods (FTIR, ¹H-NMR and SEC). The best relationship of F:V:H was 5:4:1, which allows efficient interaction with chitosan through cross-linking with borax to achieve scaffolds for potential biomedical applications. The membranes were obtained by solvent casting and analyzed by scanning electron microscopy (SEM), swelling behavior and mechanical properties. In addition, we studied the possible cytotoxicity and biocompatibility of these materials using a murine macrophage-like cell line (RAW 264.7) and bone marrow mesenchymal progenitor cells (BMPC), respectively, taking into account their intended applications. The results of this study show that the terpolymer obtained and its combination with a natural polymer is a very interesting strategy to obtain a biomaterial with possible applications in regenerative medicine and this could be extended to other structurally related systems.
format Articulo
Articulo
author Bravi Costantino, Maria Leticia
Belluzo, María Soledad
Oberti, Tamara Gisela
Cortizo, Ana María
Cortizo, María Susana
author_facet Bravi Costantino, Maria Leticia
Belluzo, María Soledad
Oberti, Tamara Gisela
Cortizo, Ana María
Cortizo, María Susana
author_sort Bravi Costantino, Maria Leticia
title Terpolymer-chitosan membranes as biomaterial
title_short Terpolymer-chitosan membranes as biomaterial
title_full Terpolymer-chitosan membranes as biomaterial
title_fullStr Terpolymer-chitosan membranes as biomaterial
title_full_unstemmed Terpolymer-chitosan membranes as biomaterial
title_sort terpolymer-chitosan membranes as biomaterial
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/164070
work_keys_str_mv AT bravicostantinomarialeticia terpolymerchitosanmembranesasbiomaterial
AT belluzomariasoledad terpolymerchitosanmembranesasbiomaterial
AT obertitamaragisela terpolymerchitosanmembranesasbiomaterial
AT cortizoanamaria terpolymerchitosanmembranesasbiomaterial
AT cortizomariasusana terpolymerchitosanmembranesasbiomaterial
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