Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering

There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was struct...

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Autores principales: Torres, María Luz, Fernández, Juan Manuel, Dellatorre, Fernando Gaspar, Cortizo, Ana María, Oberti, Tamara Gisela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/163984
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-163984
record_format dspace
spelling I19-R120-10915-1639842024-03-19T20:09:40Z http://sedici.unlp.edu.ar/handle/10915/163984 Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering Torres, María Luz Fernández, Juan Manuel Dellatorre, Fernando Gaspar Cortizo, Ana María Oberti, Tamara Gisela 2019-06 2024-03-19T13:02:55Z en Química Biología sodium alginate structural characterization purification biocompatibility cytotoxicity There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and ¹H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity. Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
spellingShingle Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
Torres, María Luz
Fernández, Juan Manuel
Dellatorre, Fernando Gaspar
Cortizo, Ana María
Oberti, Tamara Gisela
Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
topic_facet Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
description There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and ¹H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity.
format Articulo
Articulo
author Torres, María Luz
Fernández, Juan Manuel
Dellatorre, Fernando Gaspar
Cortizo, Ana María
Oberti, Tamara Gisela
author_facet Torres, María Luz
Fernández, Juan Manuel
Dellatorre, Fernando Gaspar
Cortizo, Ana María
Oberti, Tamara Gisela
author_sort Torres, María Luz
title Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_short Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_full Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_fullStr Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_full_unstemmed Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_sort purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/163984
work_keys_str_mv AT torresmarialuz purificationofalginateimprovesitsbiocompatibilityandeliminatescytotoxicityinmatrixforbonetissueengineering
AT fernandezjuanmanuel purificationofalginateimprovesitsbiocompatibilityandeliminatescytotoxicityinmatrixforbonetissueengineering
AT dellatorrefernandogaspar purificationofalginateimprovesitsbiocompatibilityandeliminatescytotoxicityinmatrixforbonetissueengineering
AT cortizoanamaria purificationofalginateimprovesitsbiocompatibilityandeliminatescytotoxicityinmatrixforbonetissueengineering
AT obertitamaragisela purificationofalginateimprovesitsbiocompatibilityandeliminatescytotoxicityinmatrixforbonetissueengineering
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