Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions

Cardiac arrhythmias are associated with various forms of heart diseases. Ventricular arrhythmias present a significant risk for sudden cardiac death. Atrial fibrillations predispose to blood clots leading to stroke and heart attack. Scientists have been developing patch-clamp technology to study ion...

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Autores principales: Chen-Izu, Ye, Hegyi, Bence, Jian, Zhong, Horvath, Balazs, Shaw, John A., Banyasz, Tamas, Izu, Leighton T.
Formato: Articulo
Lenguaje:Español
Publicado: 2023
Materias:
Ciencias Médicas
Biología
action potential
arrhythmia
cardiac myocyte
patch-clamp
electrophysiology
potencial de acción
arritmias
miocitos cardiacos
patch -clamp
electrofisiología
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/163605
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-163605
record_format dspace
spelling I19-R120-10915-1636052024-03-08T20:03:26Z http://sedici.unlp.edu.ar/handle/10915/163605 Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions Chen-Izu, Ye Hegyi, Bence Jian, Zhong Horvath, Balazs Shaw, John A. Banyasz, Tamas Izu, Leighton T. 2023-06 2024-03-08T17:08:35Z es Ciencias Médicas Biología action potential arrhythmia cardiac myocyte patch-clamp electrophysiology potencial de acción arritmias miocitos cardiacos patch -clamp electrofisiología Cardiac arrhythmias are associated with various forms of heart diseases. Ventricular arrhythmias present a significant risk for sudden cardiac death. Atrial fibrillations predispose to blood clots leading to stroke and heart attack. Scientists have been developing patch-clamp technology to study ion channels and action potentials (APs) underlying cardiac excitation and arrhythmias. Beyond the traditional patch-clamp techniques, innovative new techniques were developed for studying complex arrhythmia mechanisms. Here we review the recent development of methods including AP-Clamp, Dynamic Clamp, AP-Clamp Sequential Dissection, and Patch-Clamp-in-Gel. These methods provide powerful tools for researchers to decipher how the dynamic systems in excitation-Ca2+ signaling-contraction feedforward and feedback to control cardiac function and how their dysregulations lead to heart diseases. Las arritmias cardiacas están asociadas a diferentes tipos de enfermedad cardiaca. Las arritmias ventriculares constituyen un alto riesgo de muerte súbita. La fibrilación auricular predispone a coágulos sanguíneos que pueden producir accidentes cerebrovasculares e infarto miocárdico. Los científicos han desarrollado la técnica de patch-clamp para estudiar los canales iónicos y los potenciales de acción (PAs), que constituyen la base de la excitación y las arritmias cardiacas. Además de las clásicas técnicas de patch-clamp, se desarrollaron técnicas innovativas para estudiar los mecanismos complejos de las arritmias. En este trabajo, describimos diferentes métodos recientemente desarrollados tales como AP-clamp (“clampeo” del PA), Dynamic Clamp (“clampeo” dinámico), AP-Clamp Sequential Dissection, (disección secuencial del “clampeo” del AP), y Patch-Clamp-in-Gel (Patch clamp en gel). Estos métodos constituyen herramientas poderosas para descifrar cómo los sistemas dinámicos que constituyen la excitación-las señales de Ca2+ y la contracción, se retroalimentan para controlar la función cardiaca y cómo sus alteraciones llevan a la enfermedad cardiaca. Sociedad Argentina de Fisiología Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 22-34
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Médicas
Biología
action potential
arrhythmia
cardiac myocyte
patch-clamp
electrophysiology
potencial de acción
arritmias
miocitos cardiacos
patch -clamp
electrofisiología
spellingShingle Ciencias Médicas
Biología
action potential
arrhythmia
cardiac myocyte
patch-clamp
electrophysiology
potencial de acción
arritmias
miocitos cardiacos
patch -clamp
electrofisiología
Chen-Izu, Ye
Hegyi, Bence
Jian, Zhong
Horvath, Balazs
Shaw, John A.
Banyasz, Tamas
Izu, Leighton T.
Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
topic_facet Ciencias Médicas
Biología
action potential
arrhythmia
cardiac myocyte
patch-clamp
electrophysiology
potencial de acción
arritmias
miocitos cardiacos
patch -clamp
electrofisiología
description Cardiac arrhythmias are associated with various forms of heart diseases. Ventricular arrhythmias present a significant risk for sudden cardiac death. Atrial fibrillations predispose to blood clots leading to stroke and heart attack. Scientists have been developing patch-clamp technology to study ion channels and action potentials (APs) underlying cardiac excitation and arrhythmias. Beyond the traditional patch-clamp techniques, innovative new techniques were developed for studying complex arrhythmia mechanisms. Here we review the recent development of methods including AP-Clamp, Dynamic Clamp, AP-Clamp Sequential Dissection, and Patch-Clamp-in-Gel. These methods provide powerful tools for researchers to decipher how the dynamic systems in excitation-Ca2+ signaling-contraction feedforward and feedback to control cardiac function and how their dysregulations lead to heart diseases.
format Articulo
Articulo
author Chen-Izu, Ye
Hegyi, Bence
Jian, Zhong
Horvath, Balazs
Shaw, John A.
Banyasz, Tamas
Izu, Leighton T.
author_facet Chen-Izu, Ye
Hegyi, Bence
Jian, Zhong
Horvath, Balazs
Shaw, John A.
Banyasz, Tamas
Izu, Leighton T.
author_sort Chen-Izu, Ye
title Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
title_short Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
title_full Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
title_fullStr Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
title_full_unstemmed Innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
title_sort innovative techniques and new insights: studying cardiac ionic currents and action potentials in physiologically relevant conditions
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/163605
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