Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels

Greenhouse gas emissions, such as N2O from excessive N-fertilizer use, are of concern. Symbiotic N2-fixation by pulses such as soybean might mitigate this issue, for which inoculants carrying locally adapted Bradyrhizobium strains are recommended. In the frame of this goal, enhancing the quality con...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Brignoli, Damián, Frickel Critto, Emilia Josefina, Sandobal, Tamara Jesabel, Balda, Rocío Soledad, Castells, Cecilia Beatriz Marta, Mongiardini, Elías Javier, Pérez Giménez, Julieta, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2024
Materias:
Biología
Bradyrhizobium
soybean
chlorophyllometer
nitrogen fixation
nitrous oxide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/162302
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-162302
record_format dspace
spelling I19-R120-10915-1623022024-02-06T04:06:46Z http://sedici.unlp.edu.ar/handle/10915/162302 Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels Brignoli, Damián Frickel Critto, Emilia Josefina Sandobal, Tamara Jesabel Balda, Rocío Soledad Castells, Cecilia Beatriz Marta Mongiardini, Elías Javier Pérez Giménez, Julieta Lodeiro, Aníbal Roberto 2024-01-22 2024-02-05T15:32:44Z en Biología Bradyrhizobium soybean chlorophyllometer nitrogen fixation nitrous oxide Greenhouse gas emissions, such as N2O from excessive N-fertilizer use, are of concern. Symbiotic N2-fixation by pulses such as soybean might mitigate this issue, for which inoculants carrying locally adapted Bradyrhizobium strains are recommended. In the frame of this goal, enhancing the quality control of these inoculants is required on two key aspects: determining the presence of nosZ, for the strains being able to reduce N2O, and assessing N2- fixation potential. Previously it was demonstrated that, in soybean leaves, Ncontents are well correlated with chlorophyll contents. However, no such correlations were made with either N obtained from N2-fixation or with nodules mass, which is an indicator of nodular activity. Here we aimed to leverage the correlation between N and chlorophyll levels to develop a simple and non-destructive laboratory method to be applied in quality control of inoculants, able to assess the N2-fixing capacity of rhizobial strains. To establish such correlations, we cultivated soybeans in vermiculite with N-free nutrient solution, and inoculated them with various Bradyrhizobium field isolates that displayed a range of symbiotic N2-fixing capacities. Subsequently, we measured chlorophyll with a portable chlorophyllometer, and correlated these measures with symbiotic parameters. Moreover, we tested for the presence of nosZ by PCR. We observed significant correlations between chlorophyll and shoot nitrogen obtained from N2-fixation and, in addition, we corroborated that chlorophyll contents were significantly correlated also with nodules mass. Two B. diazoefficiens strains stood out and possessed nosZ. In contrast, B. elkanii and B. japonicum isolates displayed lower chlorophyll and symbiotic performance, and lacked nosZ. Our findings highlight the potential of measuring chlorophyll contents and testing for the presence of nosZ as two straightforward techniques that may enhance laboratory tests for quality control, enabling selection of superior and safe locally isolated strains for soybean inoculants without increased production costs. Puede accederse al conjunto de datos relacionado a este trabajo haciendo clic en "Documentos relacionados". Instituto de Biotecnología y Biología Molecular Laboratorio de Investigación y Desarrollo de Métodos Analíticos Comisión de Investigaciones Científicas de la provincia de Buenos Aires Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bradyrhizobium
soybean
chlorophyllometer
nitrogen fixation
nitrous oxide
spellingShingle Biología
Bradyrhizobium
soybean
chlorophyllometer
nitrogen fixation
nitrous oxide
Brignoli, Damián
Frickel Critto, Emilia Josefina
Sandobal, Tamara Jesabel
Balda, Rocío Soledad
Castells, Cecilia Beatriz Marta
Mongiardini, Elías Javier
Pérez Giménez, Julieta
Lodeiro, Aníbal Roberto
Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
topic_facet Biología
Bradyrhizobium
soybean
chlorophyllometer
nitrogen fixation
nitrous oxide
description Greenhouse gas emissions, such as N2O from excessive N-fertilizer use, are of concern. Symbiotic N2-fixation by pulses such as soybean might mitigate this issue, for which inoculants carrying locally adapted Bradyrhizobium strains are recommended. In the frame of this goal, enhancing the quality control of these inoculants is required on two key aspects: determining the presence of nosZ, for the strains being able to reduce N2O, and assessing N2- fixation potential. Previously it was demonstrated that, in soybean leaves, Ncontents are well correlated with chlorophyll contents. However, no such correlations were made with either N obtained from N2-fixation or with nodules mass, which is an indicator of nodular activity. Here we aimed to leverage the correlation between N and chlorophyll levels to develop a simple and non-destructive laboratory method to be applied in quality control of inoculants, able to assess the N2-fixing capacity of rhizobial strains. To establish such correlations, we cultivated soybeans in vermiculite with N-free nutrient solution, and inoculated them with various Bradyrhizobium field isolates that displayed a range of symbiotic N2-fixing capacities. Subsequently, we measured chlorophyll with a portable chlorophyllometer, and correlated these measures with symbiotic parameters. Moreover, we tested for the presence of nosZ by PCR. We observed significant correlations between chlorophyll and shoot nitrogen obtained from N2-fixation and, in addition, we corroborated that chlorophyll contents were significantly correlated also with nodules mass. Two B. diazoefficiens strains stood out and possessed nosZ. In contrast, B. elkanii and B. japonicum isolates displayed lower chlorophyll and symbiotic performance, and lacked nosZ. Our findings highlight the potential of measuring chlorophyll contents and testing for the presence of nosZ as two straightforward techniques that may enhance laboratory tests for quality control, enabling selection of superior and safe locally isolated strains for soybean inoculants without increased production costs.
format Articulo
Articulo
author Brignoli, Damián
Frickel Critto, Emilia Josefina
Sandobal, Tamara Jesabel
Balda, Rocío Soledad
Castells, Cecilia Beatriz Marta
Mongiardini, Elías Javier
Pérez Giménez, Julieta
Lodeiro, Aníbal Roberto
author_facet Brignoli, Damián
Frickel Critto, Emilia Josefina
Sandobal, Tamara Jesabel
Balda, Rocío Soledad
Castells, Cecilia Beatriz Marta
Mongiardini, Elías Javier
Pérez Giménez, Julieta
Lodeiro, Aníbal Roberto
author_sort Brignoli, Damián
title Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
title_short Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
title_full Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
title_fullStr Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
title_full_unstemmed Quality control of Bradyrhizobium inoculant strains: detection of nosZ and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
title_sort quality control of bradyrhizobium inoculant strains: detection of nosz and correlation of symbiotic efficiency with soybean leaf chlorophyll levels
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/162302
work_keys_str_mv AT brignolidamian qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT frickelcrittoemiliajosefina qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT sandobaltamarajesabel qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT baldarociosoledad qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT castellsceciliabeatrizmarta qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT mongiardinieliasjavier qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT perezgimenezjulieta qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
AT lodeiroanibalroberto qualitycontrolofbradyrhizobiuminoculantstrainsdetectionofnoszandcorrelationofsymbioticefficiencywithsoybeanleafchlorophylllevels
_version_ 1807222326872244224

Ejemplares similares