Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose

A high-fructose diet (HFD) induces murine alterations like those recorded in human prediabetes. Protective effects of isoespintanol (monoterpene isolated from Oxandra cf. xylopioides) on changes induced by HFD were evaluated. Animals were maintained for 21 days with a standard diet (C), 10% fructose...

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Autores principales: Di Sarli Gutiérrez, Luciana, Castro, María Cecilia, Farromeque Vásquez, Sherley Catherine, Villagarcía, Hernán Gonzalo, González Arbeláez, Luisa Fernanda, Rojano, Benjamín Alberto, Schinella, Guillermo Raúl, Maiztegui, Bárbara, Francini, Flavio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Ciencias Médicas
isoespintanol
hepatic lipogenesis
inflammation
oxidative stress
prediabetes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/162061
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-162061
record_format dspace
spelling I19-R120-10915-1620612023-12-28T20:07:20Z http://sedici.unlp.edu.ar/handle/10915/162061 Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose Di Sarli Gutiérrez, Luciana Castro, María Cecilia Farromeque Vásquez, Sherley Catherine Villagarcía, Hernán Gonzalo González Arbeláez, Luisa Fernanda Rojano, Benjamín Alberto Schinella, Guillermo Raúl Maiztegui, Bárbara Francini, Flavio 2023-12-28 2023-12-28T17:35:11Z en Ciencias Médicas isoespintanol hepatic lipogenesis inflammation oxidative stress prediabetes A high-fructose diet (HFD) induces murine alterations like those recorded in human prediabetes. Protective effects of isoespintanol (monoterpene isolated from Oxandra cf. xylopioides) on changes induced by HFD were evaluated. Animals were maintained for 21 days with a standard diet (C), 10% fructose (F), and F plus isoespintanol (FI, 10 mg/kg, i.p.). Glycemia, triglyceridemia, total and HDL-cholesterol, and insulin resistance index (IRX) were determined. Intraperitoneal glucose tolerance test (IGTT) was performed. In the liver, we measured glycogen, lipogenic gene expression (SREBP-1c, GPAT, FAS, and CPT1), oxidative stress (GSH and 3′-nitrotyrosine content), inflammation markers (iNOS, TNF-α, and PAI-1 gene expression; iNOS and COX-2 protein levels), p-eNOS, p-Akt, and p-GSK3β protein levels. Isoespintanol corrected enhanced triglycerides, lipogenic genes, and IRX, and reduced HDL-cholesterol induced by HFD. Increased liver glycogen and inflammatory markers and decreased GSH, p-Akt, and p-GSK3β measured in F rats were reversed by isoespintanol, and p-eNOS/e-NOS and iNOS/GADPH ratios were normalized. Isoespintanol restored glucose tolerance (IGTT) compared to F rats. These results demonstrate for the first time that isoespintanol prevents endocrine–metabolic alterations induced by HFD in prediabetic rats. These effects could be mediated by Akt/eNOS and Akt/GSK3β pathways, suggesting its possible use as a therapeutic tool for the prevention of diabetes at early stages of its development (prediabetes). Centro de Endocrinología Experimental y Aplicada Centro de Investigaciones Cardiovasculares Comisión de Investigaciones Científicas de la provincia de Buenos Aires Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
isoespintanol
hepatic lipogenesis
inflammation
oxidative stress
prediabetes
spellingShingle Ciencias Médicas
isoespintanol
hepatic lipogenesis
inflammation
oxidative stress
prediabetes
Di Sarli Gutiérrez, Luciana
Castro, María Cecilia
Farromeque Vásquez, Sherley Catherine
Villagarcía, Hernán Gonzalo
González Arbeláez, Luisa Fernanda
Rojano, Benjamín Alberto
Schinella, Guillermo Raúl
Maiztegui, Bárbara
Francini, Flavio
Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose
topic_facet Ciencias Médicas
isoespintanol
hepatic lipogenesis
inflammation
oxidative stress
prediabetes
description A high-fructose diet (HFD) induces murine alterations like those recorded in human prediabetes. Protective effects of isoespintanol (monoterpene isolated from Oxandra cf. xylopioides) on changes induced by HFD were evaluated. Animals were maintained for 21 days with a standard diet (C), 10% fructose (F), and F plus isoespintanol (FI, 10 mg/kg, i.p.). Glycemia, triglyceridemia, total and HDL-cholesterol, and insulin resistance index (IRX) were determined. Intraperitoneal glucose tolerance test (IGTT) was performed. In the liver, we measured glycogen, lipogenic gene expression (SREBP-1c, GPAT, FAS, and CPT1), oxidative stress (GSH and 3′-nitrotyrosine content), inflammation markers (iNOS, TNF-α, and PAI-1 gene expression; iNOS and COX-2 protein levels), p-eNOS, p-Akt, and p-GSK3β protein levels. Isoespintanol corrected enhanced triglycerides, lipogenic genes, and IRX, and reduced HDL-cholesterol induced by HFD. Increased liver glycogen and inflammatory markers and decreased GSH, p-Akt, and p-GSK3β measured in F rats were reversed by isoespintanol, and p-eNOS/e-NOS and iNOS/GADPH ratios were normalized. Isoespintanol restored glucose tolerance (IGTT) compared to F rats. These results demonstrate for the first time that isoespintanol prevents endocrine–metabolic alterations induced by HFD in prediabetic rats. These effects could be mediated by Akt/eNOS and Akt/GSK3β pathways, suggesting its possible use as a therapeutic tool for the prevention of diabetes at early stages of its development (prediabetes).
format Articulo
Articulo
author Di Sarli Gutiérrez, Luciana
Castro, María Cecilia
Farromeque Vásquez, Sherley Catherine
Villagarcía, Hernán Gonzalo
González Arbeláez, Luisa Fernanda
Rojano, Benjamín Alberto
Schinella, Guillermo Raúl
Maiztegui, Bárbara
Francini, Flavio
author_facet Di Sarli Gutiérrez, Luciana
Castro, María Cecilia
Farromeque Vásquez, Sherley Catherine
Villagarcía, Hernán Gonzalo
González Arbeláez, Luisa Fernanda
Rojano, Benjamín Alberto
Schinella, Guillermo Raúl
Maiztegui, Bárbara
Francini, Flavio
author_sort Di Sarli Gutiérrez, Luciana
title Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose
title_short Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose
title_full Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose
title_fullStr Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose
title_full_unstemmed Protective Effect of Monoterpene Isoespintanol in a Rat Model of Prediabetes Induced by Fructose
title_sort protective effect of monoterpene isoespintanol in a rat model of prediabetes induced by fructose
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/162061
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