Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells

Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus glo...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Rodenak-Kladniew, Boris, Castro, María Agustina, Gambaro, Rocío Celeste, Girotti, Juan Roberto, Cisneros, José Sebastián, Viña, Sonia Zulma, Padula, Gisel, Crespo, Rosana, Castro, Guillermo Raúl, Gehring, Stephan, Chain, Cecilia Yamil, Islan, Germán Abel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160996
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160996
record_format dspace
spelling I19-R120-10915-1609962023-12-01T20:07:09Z http://sedici.unlp.edu.ar/handle/10915/160996 Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells Rodenak-Kladniew, Boris Castro, María Agustina Gambaro, Rocío Celeste Girotti, Juan Roberto Cisneros, José Sebastián Viña, Sonia Zulma Padula, Gisel Crespo, Rosana Castro, Guillermo Raúl Gehring, Stephan Chain, Cecilia Yamil Islan, Germán Abel 2023 2023-12-01T14:38:19Z en Química Bioquímica essential oils solid lipid nanoparticles cancer cells biocompatibility drug delivery anticancer mechanisms Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO. Instituto de Investigaciones Bioquímicas de La Plata Instituto de Genética Veterinaria Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Centro de Investigación y Desarrollo en Criotecnología de Alimentos Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
spellingShingle Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
Rodenak-Kladniew, Boris
Castro, María Agustina
Gambaro, Rocío Celeste
Girotti, Juan Roberto
Cisneros, José Sebastián
Viña, Sonia Zulma
Padula, Gisel
Crespo, Rosana
Castro, Guillermo Raúl
Gehring, Stephan
Chain, Cecilia Yamil
Islan, Germán Abel
Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
topic_facet Química
Bioquímica
essential oils
solid lipid nanoparticles
cancer cells
biocompatibility
drug delivery
anticancer mechanisms
description Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.
format Articulo
Articulo
author Rodenak-Kladniew, Boris
Castro, María Agustina
Gambaro, Rocío Celeste
Girotti, Juan Roberto
Cisneros, José Sebastián
Viña, Sonia Zulma
Padula, Gisel
Crespo, Rosana
Castro, Guillermo Raúl
Gehring, Stephan
Chain, Cecilia Yamil
Islan, Germán Abel
author_facet Rodenak-Kladniew, Boris
Castro, María Agustina
Gambaro, Rocío Celeste
Girotti, Juan Roberto
Cisneros, José Sebastián
Viña, Sonia Zulma
Padula, Gisel
Crespo, Rosana
Castro, Guillermo Raúl
Gehring, Stephan
Chain, Cecilia Yamil
Islan, Germán Abel
author_sort Rodenak-Kladniew, Boris
title Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_short Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_full Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_fullStr Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_full_unstemmed Cytotoxic screening and enhanced anticancer activity of Lippia alba and Clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
title_sort cytotoxic screening and enhanced anticancer activity of lippia alba and clinopodium nepeta essential oils-loaded biocompatible lipid nanoparticles against lung and colon cancer cells
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160996
work_keys_str_mv AT rodenakkladniewboris cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT castromariaagustina cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT gambarorocioceleste cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT girottijuanroberto cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT cisnerosjosesebastian cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT vinasoniazulma cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT padulagisel cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT cresporosana cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT castroguillermoraul cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT gehringstephan cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT chainceciliayamil cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
AT islangermanabel cytotoxicscreeningandenhancedanticanceractivityoflippiaalbaandclinopodiumnepetaessentialoilsloadedbiocompatiblelipidnanoparticlesagainstlungandcoloncancercells
_version_ 1807222039109435392

Ejemplares similares