Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)

In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium...

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Autores principales: Tardivo Kubis, Jonás Adrián, Rodríguez, Francisco, Rossignoli, Araceli E., Riobó, Pilar, Sar, Eugenia Alicia, Sunesen, Inés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160893
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160893
record_format dspace
spelling I19-R120-10915-1608932023-11-29T20:08:28Z http://sedici.unlp.edu.ar/handle/10915/160893 Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina) Tardivo Kubis, Jonás Adrián Rodríguez, Francisco Rossignoli, Araceli E. Riobó, Pilar Sar, Eugenia Alicia Sunesen, Inés 2023 2023-11-29T17:09:04Z en Ciencias Naturales Biología Alexandrium catenella molecular analysis morphology Protoceratium reticulatum Pseudo-nitzschia multiseries toxinological profiles In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms. Facultad de Ciencias Naturales y Museo Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
spellingShingle Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
Tardivo Kubis, Jonás Adrián
Rodríguez, Francisco
Rossignoli, Araceli E.
Riobó, Pilar
Sar, Eugenia Alicia
Sunesen, Inés
Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
topic_facet Ciencias Naturales
Biología
Alexandrium catenella
molecular analysis
morphology
Protoceratium reticulatum
Pseudo-nitzschia multiseries
toxinological profiles
description In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
format Articulo
Articulo
author Tardivo Kubis, Jonás Adrián
Rodríguez, Francisco
Rossignoli, Araceli E.
Riobó, Pilar
Sar, Eugenia Alicia
Sunesen, Inés
author_facet Tardivo Kubis, Jonás Adrián
Rodríguez, Francisco
Rossignoli, Araceli E.
Riobó, Pilar
Sar, Eugenia Alicia
Sunesen, Inés
author_sort Tardivo Kubis, Jonás Adrián
title Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_short Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_full Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_fullStr Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_full_unstemmed Morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of Buenos Aires Province (Argentina)
title_sort morphological, phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of buenos aires province (argentina)
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160893
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