Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action

Background: Gomphrena perennis L. is a native plant of South America whose pharmacological properties have not been studied yet. Aim: To evaluate the cardiovascular and intestinal pharmacological effects of Gomphrena perennis L. leaves tincture (GphT) and the mechanisms involved. Experimental proc...

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Autores principales: Bonilla Bonilla, Adriana Milena, Gavilánez Buñay, Tatiana Carolina, Bayley Agnoli, Matías, Colareda, Germán Andrés, Matera, Soledad Inés, Flores, María Luján, Prieto, Julián José, Ruiz, María Esperanza, Consolini, Alicia Elvira, Ragone, María Inés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Gomphrena perennis
Antispasmodic
Blood pressure
Cardioprotection
Nitric oxide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160420
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160420
record_format dspace
spelling I19-R120-10915-1604202023-11-22T20:07:36Z http://sedici.unlp.edu.ar/handle/10915/160420 Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action Bonilla Bonilla, Adriana Milena Gavilánez Buñay, Tatiana Carolina Bayley Agnoli, Matías Colareda, Germán Andrés Matera, Soledad Inés Flores, María Luján Prieto, Julián José Ruiz, María Esperanza Consolini, Alicia Elvira Ragone, María Inés 2023 2023-11-22T17:30:08Z en Biología Gomphrena perennis Antispasmodic Blood pressure Cardioprotection Nitric oxide Background: Gomphrena perennis L. is a native plant of South America whose pharmacological properties have not been studied yet. Aim: To evaluate the cardiovascular and intestinal pharmacological effects of Gomphrena perennis L. leaves tincture (GphT) and the mechanisms involved. Experimental procedure: The chromatographic profile of GphT was done. Its ex vivo effects were evaluated by contractile concentration-response curves (CRCs) obtained from the agonist carbachol or calcium found in isolated rat small intestine, as well as in the relaxant CRCs. Cardiac effects were evaluated on isolated rat hearts exposed to ischemia/reperfusion (I/R). Experiments in vivo were performed to evaluate the diuretic activity in conscious rats and the hypotensive effect in anaesthetised rats. Results: Fifteen flavonoids were identified in GphT by HPLC-UV, including diosmin. GphT induced a noncompetitive inhibition in both carbachol and calcium CRCs on rat small intestine. The first was not affected by indomethacin. Moreover, GphT, unlike diosmin, relaxed the contracture produced by a high-potassium solution in a dose-dependently way. Neither propranolol nor L-NAME changed it. GphT did not show diuretic activity but induced hypotension insensitive to L-NAME. While GphT perfusion of isolated hearts increased injury consequent to I/R, oral administration was cardioprotective and reversed by L-NAME. However, diosmin did not improve the post-ischemic recovery. Conclusions: This study supports the use of Gomphrena perennis L. tincture as an antispasmodic and hypotensive agent. Moreover, it has been demonstrated to be preventive of post-ischemic cardiac dysfunction. However, diosmin would not be responsible for these effects. Facultad de Ciencias Exactas Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Gomphrena perennis
Antispasmodic
Blood pressure
Cardioprotection
Nitric oxide
spellingShingle Biología
Gomphrena perennis
Antispasmodic
Blood pressure
Cardioprotection
Nitric oxide
Bonilla Bonilla, Adriana Milena
Gavilánez Buñay, Tatiana Carolina
Bayley Agnoli, Matías
Colareda, Germán Andrés
Matera, Soledad Inés
Flores, María Luján
Prieto, Julián José
Ruiz, María Esperanza
Consolini, Alicia Elvira
Ragone, María Inés
Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action
topic_facet Biología
Gomphrena perennis
Antispasmodic
Blood pressure
Cardioprotection
Nitric oxide
description Background: Gomphrena perennis L. is a native plant of South America whose pharmacological properties have not been studied yet. Aim: To evaluate the cardiovascular and intestinal pharmacological effects of Gomphrena perennis L. leaves tincture (GphT) and the mechanisms involved. Experimental procedure: The chromatographic profile of GphT was done. Its ex vivo effects were evaluated by contractile concentration-response curves (CRCs) obtained from the agonist carbachol or calcium found in isolated rat small intestine, as well as in the relaxant CRCs. Cardiac effects were evaluated on isolated rat hearts exposed to ischemia/reperfusion (I/R). Experiments in vivo were performed to evaluate the diuretic activity in conscious rats and the hypotensive effect in anaesthetised rats. Results: Fifteen flavonoids were identified in GphT by HPLC-UV, including diosmin. GphT induced a noncompetitive inhibition in both carbachol and calcium CRCs on rat small intestine. The first was not affected by indomethacin. Moreover, GphT, unlike diosmin, relaxed the contracture produced by a high-potassium solution in a dose-dependently way. Neither propranolol nor L-NAME changed it. GphT did not show diuretic activity but induced hypotension insensitive to L-NAME. While GphT perfusion of isolated hearts increased injury consequent to I/R, oral administration was cardioprotective and reversed by L-NAME. However, diosmin did not improve the post-ischemic recovery. Conclusions: This study supports the use of Gomphrena perennis L. tincture as an antispasmodic and hypotensive agent. Moreover, it has been demonstrated to be preventive of post-ischemic cardiac dysfunction. However, diosmin would not be responsible for these effects.
format Articulo
Articulo
author Bonilla Bonilla, Adriana Milena
Gavilánez Buñay, Tatiana Carolina
Bayley Agnoli, Matías
Colareda, Germán Andrés
Matera, Soledad Inés
Flores, María Luján
Prieto, Julián José
Ruiz, María Esperanza
Consolini, Alicia Elvira
Ragone, María Inés
author_facet Bonilla Bonilla, Adriana Milena
Gavilánez Buñay, Tatiana Carolina
Bayley Agnoli, Matías
Colareda, Germán Andrés
Matera, Soledad Inés
Flores, María Luján
Prieto, Julián José
Ruiz, María Esperanza
Consolini, Alicia Elvira
Ragone, María Inés
author_sort Bonilla Bonilla, Adriana Milena
title Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action
title_short Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action
title_full Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action
title_fullStr Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action
title_full_unstemmed Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action
title_sort antispasmodic, cardioprotective and blood-pressure lowering properties of gomphrena perennis l. and its mechanisms of action
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160420
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