Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay

The increased use of pesticides during recent years necessitates a reevaluation of the effect of those compounds by extending the range of nontarget species commonly used in risk assessment. In the present work, we thus determined the impact of the pesticides glyphosate, carbendazim, and malathion o...

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Autores principales: Achiorno, Cecilia Luján, Villalobos, Leonor Cristina de, Ferrari, Lucrecia
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Assay medium
Bioassay
Freshwater
Infectivity
Parasites
Pesticides
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160143
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160143
record_format dspace
spelling I19-R120-10915-1601432023-11-14T20:07:28Z http://sedici.unlp.edu.ar/handle/10915/160143 Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay Achiorno, Cecilia Luján Villalobos, Leonor Cristina de Ferrari, Lucrecia 2018 2023-11-14T16:40:44Z en Biología Assay medium Bioassay Freshwater Infectivity Parasites Pesticides The increased use of pesticides during recent years necessitates a reevaluation of the effect of those compounds by extending the range of nontarget species commonly used in risk assessment. In the present work, we thus determined the impact of the pesticides glyphosate, carbendazim, and malathion on the parasite Chordodes nobilii in both natural and reconstituted freshwater as the assay medium and tested the sensitivity of three of this species's ecologically relevant parameters—e. g., embryo nonviablity and the infective capability of larvae exposed for 48 or 96 h either in ovo or after hatching via the infection index mean abundance—to compare those parameters to data from previous trials with reconstituted freshwater. In natural-freshwater assays, at environmentally relevant concentrations, all three pesticides inhibited the preparasitic-stage endpoints; with carbendazim being the most toxic pesticide and the subsequent infectivity of larvae exposed in ovo the most sensitive endpoint. In general, the 50%-inhibitory concentrations assayed in reconstituted freshwater were higher than those obtained in natural freshwater, indicating a certain protective effect; whereas the maximal toxicity of the three pesticides in both aqueous environments was essentially similar. The sensitivity of C. nobilii to these agents demonstrated that this species is one of the most susceptible to toxicity by all three pesticides. These findings with the assay methodology provide relevant information for a future assessment of the risk of toxicity to aquatic ecosystems and furthermore underscore the need to include parasitic organisms among the nontarget species canvassed. We also recommend that in the bioassays in which the risk assessment is carried out, water from a nontarget species's natural environment be used in parallel in order to obtain more conclusive results. Centro de Estudios Parasitológicos y de Vectores Articulo Preprint http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Assay medium
Bioassay
Freshwater
Infectivity
Parasites
Pesticides
spellingShingle Biología
Assay medium
Bioassay
Freshwater
Infectivity
Parasites
Pesticides
Achiorno, Cecilia Luján
Villalobos, Leonor Cristina de
Ferrari, Lucrecia
Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
topic_facet Biología
Assay medium
Bioassay
Freshwater
Infectivity
Parasites
Pesticides
description The increased use of pesticides during recent years necessitates a reevaluation of the effect of those compounds by extending the range of nontarget species commonly used in risk assessment. In the present work, we thus determined the impact of the pesticides glyphosate, carbendazim, and malathion on the parasite Chordodes nobilii in both natural and reconstituted freshwater as the assay medium and tested the sensitivity of three of this species's ecologically relevant parameters—e. g., embryo nonviablity and the infective capability of larvae exposed for 48 or 96 h either in ovo or after hatching via the infection index mean abundance—to compare those parameters to data from previous trials with reconstituted freshwater. In natural-freshwater assays, at environmentally relevant concentrations, all three pesticides inhibited the preparasitic-stage endpoints; with carbendazim being the most toxic pesticide and the subsequent infectivity of larvae exposed in ovo the most sensitive endpoint. In general, the 50%-inhibitory concentrations assayed in reconstituted freshwater were higher than those obtained in natural freshwater, indicating a certain protective effect; whereas the maximal toxicity of the three pesticides in both aqueous environments was essentially similar. The sensitivity of C. nobilii to these agents demonstrated that this species is one of the most susceptible to toxicity by all three pesticides. These findings with the assay methodology provide relevant information for a future assessment of the risk of toxicity to aquatic ecosystems and furthermore underscore the need to include parasitic organisms among the nontarget species canvassed. We also recommend that in the bioassays in which the risk assessment is carried out, water from a nontarget species's natural environment be used in parallel in order to obtain more conclusive results.
format Articulo
Preprint
author Achiorno, Cecilia Luján
Villalobos, Leonor Cristina de
Ferrari, Lucrecia
author_facet Achiorno, Cecilia Luján
Villalobos, Leonor Cristina de
Ferrari, Lucrecia
author_sort Achiorno, Cecilia Luján
title Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
title_short Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
title_full Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
title_fullStr Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
title_full_unstemmed Susceptibility of Chordodes Nobilii (Gordiida, Nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
title_sort susceptibility of chordodes nobilii (gordiida, nematomorpha) to three pesticides: influence of the water used for dilution on endpoints in an ecotoxicity bioassay
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/160143
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AT ferrarilucrecia susceptibilityofchordodesnobiliigordiidanematomorphatothreepesticidesinfluenceofthewaterusedfordilutiononendpointsinanecotoxicitybioassay
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