Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays
Gastropods are abundant key components in freshwater ecosystems, many of which are affected by anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobia parchappii is very common in the Pampean Region. The identification of native candidate species...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2023
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/160132 |
| Aporte de: |
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I19-R120-10915-160132 |
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dspace |
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Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
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SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Ecología moluscos especies nativas bioensayos dulceacuícola toxicidad aguda cloruro de cadmio mollusk native species bioassay freshwater acute toxicity cadmium chloride |
| spellingShingle |
Biología Ecología moluscos especies nativas bioensayos dulceacuícola toxicidad aguda cloruro de cadmio mollusk native species bioassay freshwater acute toxicity cadmium chloride Achiorno, Cecilia Luján Minardi, Graciela Schneider, Marcela Inés Fogel, Marilina Noelia Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays |
| topic_facet |
Biología Ecología moluscos especies nativas bioensayos dulceacuícola toxicidad aguda cloruro de cadmio mollusk native species bioassay freshwater acute toxicity cadmium chloride |
| description |
Gastropods are abundant key components in freshwater ecosystems, many of which are affected by anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobia parchappii is very common in the Pampean Region. The identification of native candidate species for toxicity testing is gaining increasing interest. The objectives of the present study were to evaluate 1) the susceptibility of H. parchappii to a reference toxicant (Cl₂ Cd), and 2) its suitability for toxicity testing by estimating LC50.
Also, we discussed the applicability of the proposed protocol. Snail sensitivity was assessed by exposure to concentrations between 0.5 and 12 mg Cd/L under acute static conditions during 72 h (exposure period). Then, snails were transferred to the control medium for 24 h (post-exposure period) and checked for recovery and those not responding to stimulation were considered to be dead. The endpoints were snail immobilization (recorded every 24 h during the exposure period) and mortality at the end of the post-exposure period. Snail sensitivity was analyzed with a generalized linear mixed model (GLMM) and LC₅₀ (lethal concentration 50%) was calculated using probit analysis. Results indicated that the number of immobilized snails increased with increasing concentration. The toxic effect of Cd on snails persisted 24 h after exposure. The LC₅₀ was 2.145 (1.675-2.641) mg Cd/L. The sensitivity of H. parchappii was similar to that reported for other freshwater standard species, supporting its suitability as test organism. The protocol was appropriate for use in toxicity testing as it is simple, inexpensive and reproducible. |
| format |
Articulo Articulo |
| author |
Achiorno, Cecilia Luján Minardi, Graciela Schneider, Marcela Inés Fogel, Marilina Noelia |
| author_facet |
Achiorno, Cecilia Luján Minardi, Graciela Schneider, Marcela Inés Fogel, Marilina Noelia |
| author_sort |
Achiorno, Cecilia Luján |
| title |
Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays |
| title_short |
Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays |
| title_full |
Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays |
| title_fullStr |
Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays |
| title_full_unstemmed |
Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays |
| title_sort |
evaluation of the south american gastropod heleobia parchappii as test organism in cadmium toxicity bioassays |
| publishDate |
2023 |
| url |
http://sedici.unlp.edu.ar/handle/10915/160132 |
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1807221835317641216 |
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I19-R120-10915-1601322023-11-14T20:07:31Z http://sedici.unlp.edu.ar/handle/10915/160132 Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays Evaluación del gasterópodo sudamericano Heleobia parchappii como organismo diagnóstico en bioensayos de toxicidad con cadmio Achiorno, Cecilia Luján Minardi, Graciela Schneider, Marcela Inés Fogel, Marilina Noelia 2023-04 2023-11-14T15:38:49Z en Biología Ecología moluscos especies nativas bioensayos dulceacuícola toxicidad aguda cloruro de cadmio mollusk native species bioassay freshwater acute toxicity cadmium chloride Gastropods are abundant key components in freshwater ecosystems, many of which are affected by anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobia parchappii is very common in the Pampean Region. The identification of native candidate species for toxicity testing is gaining increasing interest. The objectives of the present study were to evaluate 1) the susceptibility of H. parchappii to a reference toxicant (Cl₂ Cd), and 2) its suitability for toxicity testing by estimating LC50. Also, we discussed the applicability of the proposed protocol. Snail sensitivity was assessed by exposure to concentrations between 0.5 and 12 mg Cd/L under acute static conditions during 72 h (exposure period). Then, snails were transferred to the control medium for 24 h (post-exposure period) and checked for recovery and those not responding to stimulation were considered to be dead. The endpoints were snail immobilization (recorded every 24 h during the exposure period) and mortality at the end of the post-exposure period. Snail sensitivity was analyzed with a generalized linear mixed model (GLMM) and LC₅₀ (lethal concentration 50%) was calculated using probit analysis. Results indicated that the number of immobilized snails increased with increasing concentration. The toxic effect of Cd on snails persisted 24 h after exposure. The LC₅₀ was 2.145 (1.675-2.641) mg Cd/L. The sensitivity of H. parchappii was similar to that reported for other freshwater standard species, supporting its suitability as test organism. The protocol was appropriate for use in toxicity testing as it is simple, inexpensive and reproducible. Los gasterópodos son componentes clave de los ecosistemas acuáticos, y muchos de ellos están afectados por la actividad antrópica. El género Heleobia está ampliamente distribuido en la Argentina, y la especie nativa Heleobia parchappii es muy común en la Región Pampeana. Actualmente, existe un interés creciente en identificar especies nativas para usarlas en evaluaciones de toxicidad. Los objetivos de este estudio fueron evaluar 1) la susceptibilidad de H. parchappii a un tóxico de referencia (Cl₂ Cd), y 2) su idoneidad para ser utilizada en evaluaciones de toxicidad estimando la concentración letal 50% (CL₅₀). Además, discutimos la aplicabilidad del protocolo propuesto. La sensibilidad de los caracoles fue evaluada al exponerlos a concentraciones entre 0.5 y 12 mg Cd/L en un ensayo agudo bajo condiciones estáticas durante 72 h (tiempo de exposición). Los caracoles se transfirieron a un medio control durante 24 h (período de post-exposición) y se controló su estado para determinar si se recuperaban; aquellos que no respondían a los estímulos se consideraron muertos. Los puntos finales evaluados fueron la inmovilización de los caracoles (registrada cada 24 h durante el período de exposición) y la mortalidad al finalizar el período de post-exposición. La sensibilidad de los caracoles se analizó utilizando un modelo lineal generalizado y se calculó la CL₅₀ usando el análisis probit. Los resultados indican que el número de caracoles inmovilizados aumentó con el incremento de la concentración. El efecto tóxico del Cd sobre los caracoles persistió 24 h tras la exposición. La CL50 tuvo un valor de 2.145 (1.675-2.641) mg Cd/L. La sensibilidad de H. parchappii fue similar a la informada para otros especies estándar dulceacuícolas, lo que respalda su idoneidad como organismo de evaluación. El protocolo fue apropiado para su uso en evaluaciones de toxicidad ya que es simple, económico y reproducible. Centro de Estudios Parasitológicos y de Vectores Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 188-197 |