Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets

“Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maiztegui, Bárbara, Villagarcía, Hernán Gonzalo, Roman, Carolina Lisi, Flores, Luis Emilio, Prieto, José María, Castro, María Cecilia, Massa, María Laura, Schinella, Guillermo Raúl, Francini, Flavio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160006
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-160006
record_format dspace
spelling I19-R120-10915-1600062023-11-10T20:08:11Z http://sedici.unlp.edu.ar/handle/10915/160006 Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets Maiztegui, Bárbara Villagarcía, Hernán Gonzalo Roman, Carolina Lisi Flores, Luis Emilio Prieto, José María Castro, María Cecilia Massa, María Laura Schinella, Guillermo Raúl Francini, Flavio 2023 2023-11-10T12:41:25Z en Ciencias Médicas Yerba mate Ilex paraguariensis insulin secretion insulin sensitivity diabetes “Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes. Centro de Endocrinología Experimental y Aplicada Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
spellingShingle Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
Maiztegui, Bárbara
Villagarcía, Hernán Gonzalo
Roman, Carolina Lisi
Flores, Luis Emilio
Prieto, José María
Castro, María Cecilia
Massa, María Laura
Schinella, Guillermo Raúl
Francini, Flavio
Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
topic_facet Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
description “Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes.
format Articulo
Articulo
author Maiztegui, Bárbara
Villagarcía, Hernán Gonzalo
Roman, Carolina Lisi
Flores, Luis Emilio
Prieto, José María
Castro, María Cecilia
Massa, María Laura
Schinella, Guillermo Raúl
Francini, Flavio
author_facet Maiztegui, Bárbara
Villagarcía, Hernán Gonzalo
Roman, Carolina Lisi
Flores, Luis Emilio
Prieto, José María
Castro, María Cecilia
Massa, María Laura
Schinella, Guillermo Raúl
Francini, Flavio
author_sort Maiztegui, Bárbara
title Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_short Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_full Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_fullStr Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_full_unstemmed Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_sort dietary supplementation with yerba mate (ilex paraguariensis) infusion increases irs-1 and pi3k mrna levels and enhances insulin sensitivity and secretion in rat pancreatic islets
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/160006
work_keys_str_mv AT maizteguibarbara dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT villagarciahernangonzalo dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT romancarolinalisi dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT floresluisemilio dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT prietojosemaria dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT castromariacecilia dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT massamarialaura dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT schinellaguillermoraul dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
AT franciniflavio dietarysupplementationwithyerbamateilexparaguariensisinfusionincreasesirs1andpi3kmrnalevelsandenhancesinsulinsensitivityandsecretioninratpancreaticislets
_version_ 1807221811417448448

Ejemplares similares