Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli

Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of th...

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Autores principales:	Hasperué, Héctor Joaquín, Gómez Lobato, María Eugenia, Chaves, Alicia Raquel, Civello, Pedro Marcos, Martínez, Gustavo Adolfo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Ciencias Exactas Ciencias Agrarias broccoli postharvest senescence daytime chlorophyll degrading genes
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/159977
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-159977
record_format	dspace
spelling	I19-R120-10915-1599772023-11-09T20:03:36Z http://sedici.unlp.edu.ar/handle/10915/159977 Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli Hasperué, Héctor Joaquín Gómez Lobato, María Eugenia Chaves, Alicia Raquel Civello, Pedro Marcos Martínez, Gustavo Adolfo 2013-08 2023-11-09T13:45:13Z en Ciencias Exactas Ciencias Agrarias broccoli postharvest senescence daytime chlorophyll degrading genes Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of the day at which the broccoli is harvested can influence in the rate of chlorophyll degradation. This report describes changes of expression of chlorophyll degrading related genes during postharvest storage of heads harvested at different moments of day. At harvest, the content of chlorophyll did not change among samples but the expression of many genes associated with chlorophyll degradation did. The level of chlorophyll diminished during storage, but samples harvested at 18:00 h had a lower rate of degradation in comparison with heads harvested at 08:00 h. Most of genes that were previously associated with chlorophyll degradation during senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed a lower expression or a delay in their mRNA level increments in samples harvested at 18:00 h. Other genes related to chlorophyll degradation during senescence, like BoNYC or BoRCCR, showed an increased expression during senescence but there was no positive correlation between chlorophyll catabolism and expression of these genes. In conclusion, changes in the metabolism due to time of day at harvest not only influence the expression of genes during the day but also may cause different patterns of expression during postharvest. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Instituto de Fisiología Vegetal Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 22-27
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Ciencias Agrarias broccoli postharvest senescence daytime chlorophyll degrading genes
spellingShingle	Ciencias Exactas Ciencias Agrarias broccoli postharvest senescence daytime chlorophyll degrading genes Hasperué, Héctor Joaquín Gómez Lobato, María Eugenia Chaves, Alicia Raquel Civello, Pedro Marcos Martínez, Gustavo Adolfo Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
topic_facet	Ciencias Exactas Ciencias Agrarias broccoli postharvest senescence daytime chlorophyll degrading genes
description	Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of the day at which the broccoli is harvested can influence in the rate of chlorophyll degradation. This report describes changes of expression of chlorophyll degrading related genes during postharvest storage of heads harvested at different moments of day. At harvest, the content of chlorophyll did not change among samples but the expression of many genes associated with chlorophyll degradation did. The level of chlorophyll diminished during storage, but samples harvested at 18:00 h had a lower rate of degradation in comparison with heads harvested at 08:00 h. Most of genes that were previously associated with chlorophyll degradation during senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed a lower expression or a delay in their mRNA level increments in samples harvested at 18:00 h. Other genes related to chlorophyll degradation during senescence, like BoNYC or BoRCCR, showed an increased expression during senescence but there was no positive correlation between chlorophyll catabolism and expression of these genes. In conclusion, changes in the metabolism due to time of day at harvest not only influence the expression of genes during the day but also may cause different patterns of expression during postharvest.
format	Articulo Articulo
author	Hasperué, Héctor Joaquín Gómez Lobato, María Eugenia Chaves, Alicia Raquel Civello, Pedro Marcos Martínez, Gustavo Adolfo
author_facet	Hasperué, Héctor Joaquín Gómez Lobato, María Eugenia Chaves, Alicia Raquel Civello, Pedro Marcos Martínez, Gustavo Adolfo
author_sort	Hasperué, Héctor Joaquín
title	Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_short	Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_full	Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_fullStr	Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_full_unstemmed	Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_sort	time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/159977
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Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli

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