Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)

Degreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest sto...

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Autores principales: Gómez Lobato, María Eugenia, Hasperué, Héctor Joaquín, Civello, Pedro Marcos, Chaves, Alicia Raquel, Martínez, Gustavo Adolfo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/159965
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-159965
record_format dspace
spelling I19-R120-10915-1599652023-11-08T20:03:51Z http://sedici.unlp.edu.ar/handle/10915/159965 Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.) Gómez Lobato, María Eugenia Hasperué, Héctor Joaquín Civello, Pedro Marcos Chaves, Alicia Raquel Martínez, Gustavo Adolfo 2012-09 2023-11-08T18:55:23Z en Ciencias Exactas Ciencias Agrarias broccoli postharvest senescence 1-MCP chlorophyll catabolic enzyme Degreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest storage. 1-MCP treatment markedly delayed degreening and reduction of chlorophyll content. The expression of genes encoding chlorophyllases showed different behaviors. In controls, the expression of BoCLH1 decreased during postharvest storage while that of BoCLH2 increased after 72 h and then decreased. The treatment with 1-MCP did not cause any effect on BoCLH1 but induced a higher expression of BoCLH2. A high BoPPH expression level was found in senescent broccoli florets, but the up-regulation of this gene was delayed by 1-MCP treatment. The expression of BoPaO, a gene encoding a pheophorbide a oxygenase, increased after three days and then decreased. In contrast, this expression was reduced in treated broccoli. Finally, the expression of BoRCCR decreased during senescence and the treatment with 1-MCP caused a higher decrement. These results show that postharvest 1-MCP inhibit selectively some of the genes encoding enzymes related chlorophyll catabolism. Instituto de Fisiología Vegetal Centro de Investigación y Desarrollo en Criotecnología de Alimentos Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 208-211
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
spellingShingle Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
Gómez Lobato, María Eugenia
Hasperué, Héctor Joaquín
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo Adolfo
Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
topic_facet Ciencias Exactas
Ciencias Agrarias
broccoli
postharvest
senescence
1-MCP
chlorophyll catabolic enzyme
description Degreening caused by chlorophyll degradation is the most evident visual manifestation of broccoli senescence and deterioration. In this work, the effect of 1-MCP on color change, chlorophyll degradation and expression of genes associated to chlorophyll catabolism was evaluated during postharvest storage. 1-MCP treatment markedly delayed degreening and reduction of chlorophyll content. The expression of genes encoding chlorophyllases showed different behaviors. In controls, the expression of BoCLH1 decreased during postharvest storage while that of BoCLH2 increased after 72 h and then decreased. The treatment with 1-MCP did not cause any effect on BoCLH1 but induced a higher expression of BoCLH2. A high BoPPH expression level was found in senescent broccoli florets, but the up-regulation of this gene was delayed by 1-MCP treatment. The expression of BoPaO, a gene encoding a pheophorbide a oxygenase, increased after three days and then decreased. In contrast, this expression was reduced in treated broccoli. Finally, the expression of BoRCCR decreased during senescence and the treatment with 1-MCP caused a higher decrement. These results show that postharvest 1-MCP inhibit selectively some of the genes encoding enzymes related chlorophyll catabolism.
format Articulo
Articulo
author Gómez Lobato, María Eugenia
Hasperué, Héctor Joaquín
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo Adolfo
author_facet Gómez Lobato, María Eugenia
Hasperué, Héctor Joaquín
Civello, Pedro Marcos
Chaves, Alicia Raquel
Martínez, Gustavo Adolfo
author_sort Gómez Lobato, María Eugenia
title Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_short Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_full Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_fullStr Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_full_unstemmed Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)
title_sort effect of 1-mcp on the expression of chlorophyll degrading genes during senescence of broccoli (brassica oleracea l.)
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/159965
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