Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital
Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize r...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2023
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/159934 |
| Aporte de: |
| id |
I19-R120-10915-159934 |
|---|---|
| record_format |
dspace |
| spelling |
I19-R120-10915-1599342023-11-08T20:07:01Z http://sedici.unlp.edu.ar/handle/10915/159934 Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital Crivaro, Andrea Natalia Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel 2023 2023-11-08T14:22:30Z en Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik’s classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Instituto de Estudios Inmunológicos y Fisiopatológicos Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf 73-82 |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea |
| spellingShingle |
Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea Crivaro, Andrea Natalia Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| topic_facet |
Biología Clostridioides difficile Toxinotyping RFLP Virulence Antibiotic-associated diarrhea |
| description |
Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc).
Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting.
The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik’s classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins. |
| format |
Articulo Articulo |
| author |
Crivaro, Andrea Natalia Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel |
| author_facet |
Crivaro, Andrea Natalia Carasi, Paula Salto, Ileana Paula Hugo, Ayelén Amelia Soldavini Pelichotti, Paola Cecilia Bengoa, Ana Agustina Fragomeno, Melisa Serradell, María de los Ángeles Minnaard, Jessica Rolny, Ivanna Sabrina Alul, Eduardo Arregui, Leandro Fabra Martinez, Macarena E. Moreno Valero, Oscar Javier Facente, Andrea Magariños, Francisco Jewtuchowicz, Virginia Pérez, Pablo Fernando Trejo, Fernando Miguel |
| author_sort |
Crivaro, Andrea Natalia |
| title |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_short |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_full |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_fullStr |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_full_unstemmed |
Clostridioides difficile: characterization of the circulating toxinotypes in an Argentinean public hospital |
| title_sort |
clostridioides difficile: characterization of the circulating toxinotypes in an argentinean public hospital |
| publishDate |
2023 |
| url |
http://sedici.unlp.edu.ar/handle/10915/159934 |
| work_keys_str_mv |
AT crivaroandreanatalia clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT carasipaula clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT saltoileanapaula clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT hugoayelenamelia clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT soldavinipelichottipaolacecilia clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT bengoaanaagustina clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT fragomenomelisa clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT serradellmariadelosangeles clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT minnaardjessica clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT rolnyivannasabrina clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT aluleduardo clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT arreguileandro clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT fabramartinezmacarenae clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT morenovalerooscarjavier clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT facenteandrea clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT magarinosfrancisco clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT jewtuchowiczvirginia clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT perezpablofernando clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital AT trejofernandomiguel clostridioidesdifficilecharacterizationofthecirculatingtoxinotypesinanargentineanpublichospital |
| _version_ |
1807221794734604288 |