Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste

The adsorption of the lipase B from Candida antarctica (CALB) over polyethylene terephthalate (PET), polypropylene (PP), and derivatives, abundant components of urban solid waste (USW),was investigated. The characterization of the supports and biocatalysts synthesized by SEM-EDS and FTIR is presente...

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Autores principales: Llerena Suster, Carlos Rafael, Fuentes, Cynthia Analía, Sambeth, Jorge Enrique, José, Carla
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Química
Biocatálisis
Enzimas
Polietilentereftalato
Polipropileno
Bis(2-hidroxietil)tereftalato
Química verde
Profenos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/159933
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-159933
record_format dspace
spelling I19-R120-10915-1599332023-11-08T20:06:58Z http://sedici.unlp.edu.ar/handle/10915/159933 Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste Llerena Suster, Carlos Rafael Fuentes, Cynthia Analía Sambeth, Jorge Enrique José, Carla 2023-09-26 2023-11-08T14:20:34Z en Química Biocatálisis Enzimas Polietilentereftalato Polipropileno Bis(2-hidroxietil)tereftalato Química verde Profenos The adsorption of the lipase B from Candida antarctica (CALB) over polyethylene terephthalate (PET), polypropylene (PP), and derivatives, abundant components of urban solid waste (USW),was investigated. The characterization of the supports and biocatalysts synthesized by SEM-EDS and FTIR is presented. Two immobilization strategies were evaluated, conventional and total adsorption. The adsorbed protein was determined by Bradford and through high-resolution inductively coupled plasma atomic emission spectroscopy (ICP-AES). In this sense, the adsorption of CALB in all the proposed supports was evidenced, obtaining the highest protein loads in bis-(2-hydroxyethyl) terephthalate (BHET). Subsequently, the biocatalysts were applied to the esterification of rac-ibuprofen with ethanol. CALB immobilized in BHET showed remarkable activity, achieving conversions of 30%. In this context, immobilization on this support was optimized, studying the addition of sorbitol-glycerol. Thus, in the presence of 0.91 g of polyols, a catalyst with a protein load of 33.3 mg·g−1 was obtained, achieving productivity of 0.298 mmol min−1 mg−1 . Additionally, no differences were found when using BHET from USW bottles of various colors. This research shows the potential of materials derived from PET as enzymatic supports, unreported materials, that we can use as tools to achieve sustainable biotechnological applications. Centro de Investigación y Desarrollo en Ciencias Aplicadas Centro de Investigación de Proteínas Vegetales Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biocatálisis
Enzimas
Polietilentereftalato
Polipropileno
Bis(2-hidroxietil)tereftalato
Química verde
Profenos
spellingShingle Química
Biocatálisis
Enzimas
Polietilentereftalato
Polipropileno
Bis(2-hidroxietil)tereftalato
Química verde
Profenos
Llerena Suster, Carlos Rafael
Fuentes, Cynthia Analía
Sambeth, Jorge Enrique
José, Carla
Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste
topic_facet Química
Biocatálisis
Enzimas
Polietilentereftalato
Polipropileno
Bis(2-hidroxietil)tereftalato
Química verde
Profenos
description The adsorption of the lipase B from Candida antarctica (CALB) over polyethylene terephthalate (PET), polypropylene (PP), and derivatives, abundant components of urban solid waste (USW),was investigated. The characterization of the supports and biocatalysts synthesized by SEM-EDS and FTIR is presented. Two immobilization strategies were evaluated, conventional and total adsorption. The adsorbed protein was determined by Bradford and through high-resolution inductively coupled plasma atomic emission spectroscopy (ICP-AES). In this sense, the adsorption of CALB in all the proposed supports was evidenced, obtaining the highest protein loads in bis-(2-hydroxyethyl) terephthalate (BHET). Subsequently, the biocatalysts were applied to the esterification of rac-ibuprofen with ethanol. CALB immobilized in BHET showed remarkable activity, achieving conversions of 30%. In this context, immobilization on this support was optimized, studying the addition of sorbitol-glycerol. Thus, in the presence of 0.91 g of polyols, a catalyst with a protein load of 33.3 mg·g−1 was obtained, achieving productivity of 0.298 mmol min−1 mg−1 . Additionally, no differences were found when using BHET from USW bottles of various colors. This research shows the potential of materials derived from PET as enzymatic supports, unreported materials, that we can use as tools to achieve sustainable biotechnological applications.
format Articulo
Articulo
author Llerena Suster, Carlos Rafael
Fuentes, Cynthia Analía
Sambeth, Jorge Enrique
José, Carla
author_facet Llerena Suster, Carlos Rafael
Fuentes, Cynthia Analía
Sambeth, Jorge Enrique
José, Carla
author_sort Llerena Suster, Carlos Rafael
title Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste
title_short Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste
title_full Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste
title_fullStr Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste
title_full_unstemmed Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste
title_sort immobilization of the lipase b from candida antarctica on urban solid waste
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/159933
work_keys_str_mv AT llerenasustercarlosrafael immobilizationofthelipasebfromcandidaantarcticaonurbansolidwaste
AT fuentescynthiaanalia immobilizationofthelipasebfromcandidaantarcticaonurbansolidwaste
AT sambethjorgeenrique immobilizationofthelipasebfromcandidaantarcticaonurbansolidwaste
AT josecarla immobilizationofthelipasebfromcandidaantarcticaonurbansolidwaste
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