Role of glucocorticoid receptor (GR) in white adipose tissue beiging

Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-dam...

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Autores principales: Martín, Florencia M., Alzamendi, Ana, Harnichar, Alejandro Ezequiel, Castrogiovanni, Daniel Cayetano, Zubiría, María Guillermina, Spinedi, Eduardo Julio, Giovambattista, Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/159808
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-159808
record_format dspace
spelling I19-R120-10915-1598082023-11-06T20:06:59Z http://sedici.unlp.edu.ar/handle/10915/159808 Role of glucocorticoid receptor (GR) in white adipose tissue beiging Martín, Florencia M. Alzamendi, Ana Harnichar, Alejandro Ezequiel Castrogiovanni, Daniel Cayetano Zubiría, María Guillermina Spinedi, Eduardo Julio Giovambattista, Andrés 2023 2023-11-06T13:23:50Z en Biología Glucocorticoid excess Monosodim L-glutamate Obesity White adiposity browning Non-shivering thermogenesis Adrenalectomy Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes. Centro de Endocrinología Experimental y Aplicada Instituto Multidisciplinario de Biología Celular Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
spellingShingle Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
Martín, Florencia M.
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Castrogiovanni, Daniel Cayetano
Zubiría, María Guillermina
Spinedi, Eduardo Julio
Giovambattista, Andrés
Role of glucocorticoid receptor (GR) in white adipose tissue beiging
topic_facet Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
description Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes.
format Articulo
Articulo
author Martín, Florencia M.
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Castrogiovanni, Daniel Cayetano
Zubiría, María Guillermina
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_facet Martín, Florencia M.
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Castrogiovanni, Daniel Cayetano
Zubiría, María Guillermina
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_sort Martín, Florencia M.
title Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_short Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_full Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_fullStr Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_full_unstemmed Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_sort role of glucocorticoid receptor (gr) in white adipose tissue beiging
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/159808
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