Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors

Breast cancer spreading to different organs have been related to different molecules and mechanisms, but cutaneous metastasis remains unexplored. Increasing evidence showed that MUC1 and some of its carbohydrate associated antigens may be implicated in breast cancer metastasis. In this study we anal...

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Autores principales: Luna, Amalia, Rabassa, Martín Enrique, Isla Larrain, Marina Teresita, Cabaleiro, P., Zwenger, Ariel Osvaldo, Canzoneri, Romina, Segal-Eiras, Amada, Abba, Martín Carlos, Croce, María Virginia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
Breast cancer
Cutaneous metastasis
uMUC1
sLex
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/159663
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-159663
record_format dspace
spelling I19-R120-10915-1596632023-11-01T20:07:25Z http://sedici.unlp.edu.ar/handle/10915/159663 Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors Luna, Amalia Rabassa, Martín Enrique Isla Larrain, Marina Teresita Cabaleiro, P. Zwenger, Ariel Osvaldo Canzoneri, Romina Segal-Eiras, Amada Abba, Martín Carlos Croce, María Virginia 2020-06-15 2023-11-01T14:50:36Z en Ciencias Médicas Breast cancer Cutaneous metastasis uMUC1 sLex Breast cancer spreading to different organs have been related to different molecules and mechanisms, but cutaneous metastasis remains unexplored. Increasing evidence showed that MUC1 and some of its carbohydrate associated antigens may be implicated in breast cancer metastasis. In this study we analyzed these tumor markers in order to identify breast cancer cutaneous metastatic profiles. A cohort of 26 primary tumors from breast cancer patients with cutaneous metastases were included; also, cutaneous and lymphatic node metastatic samples and primary tumors from breast cancer patients without metastases were analysed. Immunohistochemical (IHC) studies demonstrated that both underglycosylated MUC1 (uMUC1) and sialyl Lewis x (sLex) to be positively associated with cutaneous metastatic primary tumors (p < 0.05). Notably, a high percentage of tumors with cutaneous metastases were characterized as triple negative and Her2+ tumors (37.5 % and 29 %, respectively). Some discordant results were found between primary tumors and their matched cutaneous metastases. To determine if MUC1 variants may be carriers of carbohydrate antigens, subcellular fractions from a cutaneous metastatic lesion were obtained, immunoprecipitated and analyzed by Western blot. We found that the isolated uMUC1 with a molecular weight of>200 kDa was also the site for binding of antisLex MAb; in coincidence, a high correlation of positive IHC expression of both markers was observed. Our findings confirm that breast cancer cutaneous metastases were associated to highly malignant primary tumors and sustain the hypothesis that u-MUC1 and sLe x may drive breast cancer cutaneous metastases. Centro de Investigaciones Inmunológicas Básicas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Breast cancer
Cutaneous metastasis
uMUC1
sLex
spellingShingle Ciencias Médicas
Breast cancer
Cutaneous metastasis
uMUC1
sLex
Luna, Amalia
Rabassa, Martín Enrique
Isla Larrain, Marina Teresita
Cabaleiro, P.
Zwenger, Ariel Osvaldo
Canzoneri, Romina
Segal-Eiras, Amada
Abba, Martín Carlos
Croce, María Virginia
Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors
topic_facet Ciencias Médicas
Breast cancer
Cutaneous metastasis
uMUC1
sLex
description Breast cancer spreading to different organs have been related to different molecules and mechanisms, but cutaneous metastasis remains unexplored. Increasing evidence showed that MUC1 and some of its carbohydrate associated antigens may be implicated in breast cancer metastasis. In this study we analyzed these tumor markers in order to identify breast cancer cutaneous metastatic profiles. A cohort of 26 primary tumors from breast cancer patients with cutaneous metastases were included; also, cutaneous and lymphatic node metastatic samples and primary tumors from breast cancer patients without metastases were analysed. Immunohistochemical (IHC) studies demonstrated that both underglycosylated MUC1 (uMUC1) and sialyl Lewis x (sLex) to be positively associated with cutaneous metastatic primary tumors (p < 0.05). Notably, a high percentage of tumors with cutaneous metastases were characterized as triple negative and Her2+ tumors (37.5 % and 29 %, respectively). Some discordant results were found between primary tumors and their matched cutaneous metastases. To determine if MUC1 variants may be carriers of carbohydrate antigens, subcellular fractions from a cutaneous metastatic lesion were obtained, immunoprecipitated and analyzed by Western blot. We found that the isolated uMUC1 with a molecular weight of>200 kDa was also the site for binding of antisLex MAb; in coincidence, a high correlation of positive IHC expression of both markers was observed. Our findings confirm that breast cancer cutaneous metastases were associated to highly malignant primary tumors and sustain the hypothesis that u-MUC1 and sLe x may drive breast cancer cutaneous metastases.
format Articulo
Articulo
author Luna, Amalia
Rabassa, Martín Enrique
Isla Larrain, Marina Teresita
Cabaleiro, P.
Zwenger, Ariel Osvaldo
Canzoneri, Romina
Segal-Eiras, Amada
Abba, Martín Carlos
Croce, María Virginia
author_facet Luna, Amalia
Rabassa, Martín Enrique
Isla Larrain, Marina Teresita
Cabaleiro, P.
Zwenger, Ariel Osvaldo
Canzoneri, Romina
Segal-Eiras, Amada
Abba, Martín Carlos
Croce, María Virginia
author_sort Luna, Amalia
title Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors
title_short Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors
title_full Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors
title_fullStr Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors
title_full_unstemmed Breast cancer cutaneous metastases are associated to uMUC1 and sialyl Lewis x and to highly malignant primary tumors
title_sort breast cancer cutaneous metastases are associated to umuc1 and sialyl lewis x and to highly malignant primary tumors
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/159663
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