Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium

Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been po...

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Autores principales: Nieto, Esteban Emanuel, Macchi, Marianela, Valacco, María P., Festa, Sabrina, Morelli, Irma Susana, Coppotelli, Bibiana Marina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/157726
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-157726
record_format dspace
spelling I19-R120-10915-1577262023-09-16T04:06:16Z http://sedici.unlp.edu.ar/handle/10915/157726 Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium Nieto, Esteban Emanuel Macchi, Marianela Valacco, María P. Festa, Sabrina Morelli, Irma Susana Coppotelli, Bibiana Marina 2023 2023-09-15T17:41:29Z en Biología Química Synthetic bacterial consortium Phenanthrene (PHN) degradation pathway Microbial degradation potential Bioaugmentation Bioremediation Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation. Centro de Investigación y Desarrollo en Fermentaciones Industriales Universidad de Buenos Aires Comisión de Investigaciones Científicas de la provincia de Buenos Aires Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 181-197
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
spellingShingle Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
Nieto, Esteban Emanuel
Macchi, Marianela
Valacco, María P.
Festa, Sabrina
Morelli, Irma Susana
Coppotelli, Bibiana Marina
Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
topic_facet Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
description Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.
format Articulo
Articulo
author Nieto, Esteban Emanuel
Macchi, Marianela
Valacco, María P.
Festa, Sabrina
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author_facet Nieto, Esteban Emanuel
Macchi, Marianela
Valacco, María P.
Festa, Sabrina
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author_sort Nieto, Esteban Emanuel
title Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_short Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_full Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_fullStr Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_full_unstemmed Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_sort metaproteomic and gene expression analysis of interspecies interactions in a pah‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/157726
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