Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe...

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Autores principales: Corrêa De Melo, Thatiana, Trevisan Silva, Dilza, Alvarez Flores, Miryam P., Nascimento Gomes, Renata, Medina De Souza, Marcelo, Valerio, Hellen Paula, Oliveira, Douglas S., DeOcesano Pereira, Carlos, Fongaro Botosso, Viviane, Calil Jorge, Soraia Attie, Schattner, Mirta, Gómez, Ricardo Martín, Chudzinski Tavassi, Ana Marisa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156970
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156970
record_format dspace
spelling I19-R120-10915-1569702023-08-28T20:06:28Z http://sedici.unlp.edu.ar/handle/10915/156970 Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells Corrêa De Melo, Thatiana Trevisan Silva, Dilza Alvarez Flores, Miryam P. Nascimento Gomes, Renata Medina De Souza, Marcelo Valerio, Hellen Paula Oliveira, Douglas S. DeOcesano Pereira, Carlos Fongaro Botosso, Viviane Calil Jorge, Soraia Attie Schattner, Mirta Gómez, Ricardo Martín Chudzinski Tavassi, Ana Marisa 2022-09 2023-08-28T18:27:32Z en Ciencias Exactas Biología SARS-CoV-2 endothelial cells proteomics mass spectrometry HUVECs Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction. Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
spellingShingle Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
Corrêa De Melo, Thatiana
Trevisan Silva, Dilza
Alvarez Flores, Miryam P.
Nascimento Gomes, Renata
Medina De Souza, Marcelo
Valerio, Hellen Paula
Oliveira, Douglas S.
DeOcesano Pereira, Carlos
Fongaro Botosso, Viviane
Calil Jorge, Soraia Attie
Schattner, Mirta
Gómez, Ricardo Martín
Chudzinski Tavassi, Ana Marisa
Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
topic_facet Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction.
format Articulo
Articulo
author Corrêa De Melo, Thatiana
Trevisan Silva, Dilza
Alvarez Flores, Miryam P.
Nascimento Gomes, Renata
Medina De Souza, Marcelo
Valerio, Hellen Paula
Oliveira, Douglas S.
DeOcesano Pereira, Carlos
Fongaro Botosso, Viviane
Calil Jorge, Soraia Attie
Schattner, Mirta
Gómez, Ricardo Martín
Chudzinski Tavassi, Ana Marisa
author_facet Corrêa De Melo, Thatiana
Trevisan Silva, Dilza
Alvarez Flores, Miryam P.
Nascimento Gomes, Renata
Medina De Souza, Marcelo
Valerio, Hellen Paula
Oliveira, Douglas S.
DeOcesano Pereira, Carlos
Fongaro Botosso, Viviane
Calil Jorge, Soraia Attie
Schattner, Mirta
Gómez, Ricardo Martín
Chudzinski Tavassi, Ana Marisa
author_sort Corrêa De Melo, Thatiana
title Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_short Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_full Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_fullStr Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_full_unstemmed Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_sort proteomic analysis identifies molecular players and biological processes specific to sars-cov-2 exposure in endothelial cells
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/156970
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