Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells

Wild mushrooms have gained great importance for being a source of biologically active compounds. In this work, we evaluate the anticancer and antioxidant activity of a water-soluble crude polysaccharide extract isolated from the fruiting bodies of the Ganoderma aff. australe (GACP). This mushroom wa...

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Autores principales: Muñoz Castiblanco, Tatiana, Santa Maria de la Parra, Lucía, Peña Cañón, Rocio, Mejía Giraldo, Juan Camilo, León, Ignacio Esteban, Puertas Mejía, Miguel Ángel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Química
anticancer activity
antioxidant activity
G. aff. australe
MG-63 cells
mushrooms
osteosarcoma
polysaccharides
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156295
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156295
record_format dspace
spelling I19-R120-10915-1562952023-08-14T20:08:21Z http://sedici.unlp.edu.ar/handle/10915/156295 Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells Muñoz Castiblanco, Tatiana Santa Maria de la Parra, Lucía Peña Cañón, Rocio Mejía Giraldo, Juan Camilo León, Ignacio Esteban Puertas Mejía, Miguel Ángel 2022 2023-08-14T15:40:36Z en Química anticancer activity antioxidant activity G. aff. australe MG-63 cells mushrooms osteosarcoma polysaccharides Wild mushrooms have gained great importance for being a source of biologically active compounds. In this work, we evaluate the anticancer and antioxidant activity of a water-soluble crude polysaccharide extract isolated from the fruiting bodies of the Ganoderma aff. australe (GACP). This mushroom was collected in San Mateo (Boyacá, Colombia) and identified based on macroscopic and microscopic characterization. GACP was characterized by UV–Vis spectroscopy, Fourier-transform infrared spectroscopy, high-performance liquid chromatography–diode array detector, and nuclear magnetic resonance. The antiradical and antioxidant activity were evaluated by different methods and its anticancer activity was verified in the osteosarcoma MG-63 human cell line. Chemical and spectroscopic analysis indicated that GACP consisted of β-D-Glcp-(1→, →3)-β-D-Glcp-(1→ and α-D-Glcp-(1→ residues. The results of the biological activity showed that GACP exhibited high antioxidant activity in the different methods and models studied. Moreover, the results showed that GACP impaired cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay) and cell proliferation (clonogenic assay) in a dose–response manner on MG-63 cells. The findings of this work promote the use of mushroom-derived compounds as anticancer and antioxidant agents for potential use in the pharmaceutical and food industries. Centro de Química Inorgánica Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
anticancer activity
antioxidant activity
G. aff. australe
MG-63 cells
mushrooms
osteosarcoma
polysaccharides
spellingShingle Química
anticancer activity
antioxidant activity
G. aff. australe
MG-63 cells
mushrooms
osteosarcoma
polysaccharides
Muñoz Castiblanco, Tatiana
Santa Maria de la Parra, Lucía
Peña Cañón, Rocio
Mejía Giraldo, Juan Camilo
León, Ignacio Esteban
Puertas Mejía, Miguel Ángel
Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells
topic_facet Química
anticancer activity
antioxidant activity
G. aff. australe
MG-63 cells
mushrooms
osteosarcoma
polysaccharides
description Wild mushrooms have gained great importance for being a source of biologically active compounds. In this work, we evaluate the anticancer and antioxidant activity of a water-soluble crude polysaccharide extract isolated from the fruiting bodies of the Ganoderma aff. australe (GACP). This mushroom was collected in San Mateo (Boyacá, Colombia) and identified based on macroscopic and microscopic characterization. GACP was characterized by UV–Vis spectroscopy, Fourier-transform infrared spectroscopy, high-performance liquid chromatography–diode array detector, and nuclear magnetic resonance. The antiradical and antioxidant activity were evaluated by different methods and its anticancer activity was verified in the osteosarcoma MG-63 human cell line. Chemical and spectroscopic analysis indicated that GACP consisted of β-D-Glcp-(1→, →3)-β-D-Glcp-(1→ and α-D-Glcp-(1→ residues. The results of the biological activity showed that GACP exhibited high antioxidant activity in the different methods and models studied. Moreover, the results showed that GACP impaired cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay) and cell proliferation (clonogenic assay) in a dose–response manner on MG-63 cells. The findings of this work promote the use of mushroom-derived compounds as anticancer and antioxidant agents for potential use in the pharmaceutical and food industries.
format Articulo
Articulo
author Muñoz Castiblanco, Tatiana
Santa Maria de la Parra, Lucía
Peña Cañón, Rocio
Mejía Giraldo, Juan Camilo
León, Ignacio Esteban
Puertas Mejía, Miguel Ángel
author_facet Muñoz Castiblanco, Tatiana
Santa Maria de la Parra, Lucía
Peña Cañón, Rocio
Mejía Giraldo, Juan Camilo
León, Ignacio Esteban
Puertas Mejía, Miguel Ángel
author_sort Muñoz Castiblanco, Tatiana
title Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells
title_short Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells
title_full Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells
title_fullStr Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells
title_full_unstemmed Anticancer and Antioxidant Activity of Water-Soluble Polysaccharides from Ganoderma aff. australe against Human Osteosarcoma Cells
title_sort anticancer and antioxidant activity of water-soluble polysaccharides from ganoderma aff. australe against human osteosarcoma cells
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/156295
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