Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry

In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American...

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Autores principales: Rimondi, Agustina, Olivera, Valeria S., Soria, Ivana, Parisi, Gustavo Daniel, Rumbo, Martín, Pérez, Daniel R.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/155926
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-155926
record_format dspace
spelling I19-R120-10915-1559262023-08-04T20:07:47Z http://sedici.unlp.edu.ar/handle/10915/155926 Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry Rimondi, Agustina Olivera, Valeria S. Soria, Ivana Parisi, Gustavo Daniel Rumbo, Martín Pérez, Daniel R. 2022 2023-08-04T17:14:38Z en Biología Ciencias Veterinarias influenza A virus South American IAV viral adaptation viral fitness viral transmission chicken studies risk to poultry immunopathogenesis In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain. Instituto de Estudios Inmunológicos y Fisiopatológicos Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
spellingShingle Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
Rimondi, Agustina
Olivera, Valeria S.
Soria, Ivana
Parisi, Gustavo Daniel
Rumbo, Martín
Pérez, Daniel R.
Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
topic_facet Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
description In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.
format Articulo
Articulo
author Rimondi, Agustina
Olivera, Valeria S.
Soria, Ivana
Parisi, Gustavo Daniel
Rumbo, Martín
Pérez, Daniel R.
author_facet Rimondi, Agustina
Olivera, Valeria S.
Soria, Ivana
Parisi, Gustavo Daniel
Rumbo, Martín
Pérez, Daniel R.
author_sort Rimondi, Agustina
title Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_short Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_full Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_fullStr Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_full_unstemmed Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_sort few amino acid mutations in h6 influenza a virus from south american lineage increase viral replication efficiency in poultry
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/155926
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