Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach

To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as p...

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Detalles Bibliográficos
Autores principales: Solmi, Leandro, Rosli, Hernán Guillermo, Pombo, Marina Alejandra, Stalder, Santiago, Rossi, Franco R., Romero, Fernando M., Ruiz, Oscar A., Gárriz, Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Biología
putrescine
spermidine
secondary metabolism
plant pathogen
Pseudomonas syringae
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/155923
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-155923
record_format dspace
spelling I19-R120-10915-1559232023-08-04T20:04:45Z http://sedici.unlp.edu.ar/handle/10915/155923 Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach Solmi, Leandro Rosli, Hernán Guillermo Pombo, Marina Alejandra Stalder, Santiago Rossi, Franco R. Romero, Fernando M. Ruiz, Oscar A. Gárriz, Andrés 2022 2023-08-04T17:06:40Z en Biología putrescine spermidine secondary metabolism plant pathogen Pseudomonas syringae To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as polyamines have attracted considerable attention as they are involved in important cellular processes, but it is not known yet how phytopathogenic bacteria regulate polyamine homeostasis in the plant environment. In the present study, we performed a meta-analysis of publicly available transcriptomic data from experiments conducted on bacteria to begin delving into this topic and better understand the regulation of polyamine metabolism and its links to pathogenicity. We focused our research on Pseudomonas syringae, an important phytopathogen that causes disease in many economically valuable plant species. Our analysis discovered that polyamine synthesis, as well as general gene expression activation and energy production are induced in the early stages of the disease. On the contrary, synthesis of these compounds is inhibited whereas its transport is upregulated later in the process, which correlates with the induction of virulence genes and the metabolism of nitrogen and carboxylic acids. We also found that activation of plant defense mechanisms affects bacterial polyamine synthesis to some extent, which could reduce bacterial cell fitness in the plant environment. Furthermore, data suggest that a proper bacterial response to oxidative conditions requires a decrease in polyamine production. The implications of these findings are discussed. Instituto de Fisiología Vegetal Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
putrescine
spermidine
secondary metabolism
plant pathogen
Pseudomonas syringae
spellingShingle Biología
putrescine
spermidine
secondary metabolism
plant pathogen
Pseudomonas syringae
Solmi, Leandro
Rosli, Hernán Guillermo
Pombo, Marina Alejandra
Stalder, Santiago
Rossi, Franco R.
Romero, Fernando M.
Ruiz, Oscar A.
Gárriz, Andrés
Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
topic_facet Biología
putrescine
spermidine
secondary metabolism
plant pathogen
Pseudomonas syringae
description To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as polyamines have attracted considerable attention as they are involved in important cellular processes, but it is not known yet how phytopathogenic bacteria regulate polyamine homeostasis in the plant environment. In the present study, we performed a meta-analysis of publicly available transcriptomic data from experiments conducted on bacteria to begin delving into this topic and better understand the regulation of polyamine metabolism and its links to pathogenicity. We focused our research on Pseudomonas syringae, an important phytopathogen that causes disease in many economically valuable plant species. Our analysis discovered that polyamine synthesis, as well as general gene expression activation and energy production are induced in the early stages of the disease. On the contrary, synthesis of these compounds is inhibited whereas its transport is upregulated later in the process, which correlates with the induction of virulence genes and the metabolism of nitrogen and carboxylic acids. We also found that activation of plant defense mechanisms affects bacterial polyamine synthesis to some extent, which could reduce bacterial cell fitness in the plant environment. Furthermore, data suggest that a proper bacterial response to oxidative conditions requires a decrease in polyamine production. The implications of these findings are discussed.
format Articulo
Articulo
author Solmi, Leandro
Rosli, Hernán Guillermo
Pombo, Marina Alejandra
Stalder, Santiago
Rossi, Franco R.
Romero, Fernando M.
Ruiz, Oscar A.
Gárriz, Andrés
author_facet Solmi, Leandro
Rosli, Hernán Guillermo
Pombo, Marina Alejandra
Stalder, Santiago
Rossi, Franco R.
Romero, Fernando M.
Ruiz, Oscar A.
Gárriz, Andrés
author_sort Solmi, Leandro
title Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_short Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_full Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_fullStr Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_full_unstemmed Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_sort inferring the significance of the polyamine metabolism in the phytopathogenic bacteria pseudomonas syringae: a meta-analysis approach
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/155923
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