Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability

Head and neck squamous cell carcinoma (HNSCC) is a remarkably heterogeneous disease with around 50% mortality, a fact that has prompted researchers to try new approaches to improve patient survival. Hemoxygenase-1 (HO-1) is the rate-limiting step for heme degradation into carbon monoxide, free iron...

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Autores principales: Mascaró, Marilina, Alonso, Exequiel G., Schweitzer, Karen, Rabassa, Martín Enrique, Carballido, Jessica Andrea, Ibarra, Agustina, Alonso, Eliana N., Bermúdez, Vicente, Fernández Chavez, Lucía, Coló, Georgina P., Ferronato, María Julia, Pichel, Pamela, Recio, Sergio, Clemente, Valentina, Fermento, María Eugenia, Facchinetti, María Marta, Curino, Alejandro C.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Medicina
Biología
hemoxygenase-1
nucleus
cancer
head and neck
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/155707
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-155707
record_format dspace
spelling I19-R120-10915-1557072023-08-02T04:06:43Z http://sedici.unlp.edu.ar/handle/10915/155707 Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability Mascaró, Marilina Alonso, Exequiel G. Schweitzer, Karen Rabassa, Martín Enrique Carballido, Jessica Andrea Ibarra, Agustina Alonso, Eliana N. Bermúdez, Vicente Fernández Chavez, Lucía Coló, Georgina P. Ferronato, María Julia Pichel, Pamela Recio, Sergio Clemente, Valentina Fermento, María Eugenia Facchinetti, María Marta Curino, Alejandro C. 2022-10 2023-08-01T15:37:37Z en Medicina Biología hemoxygenase-1 nucleus cancer head and neck Head and neck squamous cell carcinoma (HNSCC) is a remarkably heterogeneous disease with around 50% mortality, a fact that has prompted researchers to try new approaches to improve patient survival. Hemoxygenase-1 (HO-1) is the rate-limiting step for heme degradation into carbon monoxide, free iron and biliverdin. We have previously reported that HO-1 protein is upregulated in human HNSCC samples and that it is localized in the cytoplasmic and nuclear compartments; additionally, we have demonstrated that HO-1 nuclear localization is associated with malignant progression. In this work, by using pharmacological and genetic experimental approaches, we begin to elucidate the mechanisms through which HO-1 plays a role in HNSCC. We found that high HO-1 mRNA was associated with decreased patient survival in early stages of HNSCC. In vitro experiments have shown that full-length HO-1 localizes in the cytoplasm, and that, depending on its enzymatic activity, it increases cell viability and promotes cell cycle progression. Instead, HO-1 does not alter migration capacity. Furthermore, we show that C-terminal truncated HO-1 localizes into the nucleus, increases cell viability and promotes cell cycle progression. In conclusion, we herein demonstrate that HO-1 displays protumor activities in HNSCC that depend, at least in part, on the nuclear localization of HO-1. Centro de Investigaciones Inmunológicas Básicas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Biología
hemoxygenase-1
nucleus
cancer
head and neck
spellingShingle Medicina
Biología
hemoxygenase-1
nucleus
cancer
head and neck
Mascaró, Marilina
Alonso, Exequiel G.
Schweitzer, Karen
Rabassa, Martín Enrique
Carballido, Jessica Andrea
Ibarra, Agustina
Alonso, Eliana N.
Bermúdez, Vicente
Fernández Chavez, Lucía
Coló, Georgina P.
Ferronato, María Julia
Pichel, Pamela
Recio, Sergio
Clemente, Valentina
Fermento, María Eugenia
Facchinetti, María Marta
Curino, Alejandro C.
Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability
topic_facet Medicina
Biología
hemoxygenase-1
nucleus
cancer
head and neck
description Head and neck squamous cell carcinoma (HNSCC) is a remarkably heterogeneous disease with around 50% mortality, a fact that has prompted researchers to try new approaches to improve patient survival. Hemoxygenase-1 (HO-1) is the rate-limiting step for heme degradation into carbon monoxide, free iron and biliverdin. We have previously reported that HO-1 protein is upregulated in human HNSCC samples and that it is localized in the cytoplasmic and nuclear compartments; additionally, we have demonstrated that HO-1 nuclear localization is associated with malignant progression. In this work, by using pharmacological and genetic experimental approaches, we begin to elucidate the mechanisms through which HO-1 plays a role in HNSCC. We found that high HO-1 mRNA was associated with decreased patient survival in early stages of HNSCC. In vitro experiments have shown that full-length HO-1 localizes in the cytoplasm, and that, depending on its enzymatic activity, it increases cell viability and promotes cell cycle progression. Instead, HO-1 does not alter migration capacity. Furthermore, we show that C-terminal truncated HO-1 localizes into the nucleus, increases cell viability and promotes cell cycle progression. In conclusion, we herein demonstrate that HO-1 displays protumor activities in HNSCC that depend, at least in part, on the nuclear localization of HO-1.
format Articulo
Articulo
author Mascaró, Marilina
Alonso, Exequiel G.
Schweitzer, Karen
Rabassa, Martín Enrique
Carballido, Jessica Andrea
Ibarra, Agustina
Alonso, Eliana N.
Bermúdez, Vicente
Fernández Chavez, Lucía
Coló, Georgina P.
Ferronato, María Julia
Pichel, Pamela
Recio, Sergio
Clemente, Valentina
Fermento, María Eugenia
Facchinetti, María Marta
Curino, Alejandro C.
author_facet Mascaró, Marilina
Alonso, Exequiel G.
Schweitzer, Karen
Rabassa, Martín Enrique
Carballido, Jessica Andrea
Ibarra, Agustina
Alonso, Eliana N.
Bermúdez, Vicente
Fernández Chavez, Lucía
Coló, Georgina P.
Ferronato, María Julia
Pichel, Pamela
Recio, Sergio
Clemente, Valentina
Fermento, María Eugenia
Facchinetti, María Marta
Curino, Alejandro C.
author_sort Mascaró, Marilina
title Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability
title_short Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability
title_full Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability
title_fullStr Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability
title_full_unstemmed Hemoxygenase-1 Promotes Head and Neck Cancer Cell Viability
title_sort hemoxygenase-1 promotes head and neck cancer cell viability
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/155707
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