Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat

The aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live la...

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Autores principales: Lavarías, Sabrina María Luisa, Arrighetti, Florencia, Landro, Sonia Maribel, Delevati Colpo, Karine
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/155695
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-155695
record_format dspace
spelling I19-R120-10915-1556952023-08-02T04:06:49Z http://sedici.unlp.edu.ar/handle/10915/155695 Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat Lavarías, Sabrina María Luisa Arrighetti, Florencia Landro, Sonia Maribel Delevati Colpo, Karine 2022-12 2023-08-01T14:13:37Z en Ciencias Naturales aquatic toxicology histopathology LC₅₀ oxidative stress prawn spirotetramat The aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live larvae were fixed for histological analysis. In addition, ovigerous females were exposed to a sublethal concentration of STM (1.7 mg/L) for 96 h to evaluate the activity of the enzymes catalase, glutathione-S-transferase, and superoxide dismutase as well as the lipoperoxidation (LPO) and protein oxidation levels in embryos. The larvae showed a high sensitivity to STM evidenced by the LC₅₀-96 h value (0.011 mg/L). On the contrary, the embryos were highly resistant to STM exposure, and no lethal effect was observed in the treatments with high concentrations of this insecticide (LC₅₀-96 h > 150 mg/L). Among all the biochemical parameters evaluated in the embryos exposed to STM, only LPO showed a significant increase compared to controls. This was probably due to a restricted entry of the insecticide through the embryonic coat. Thus, a preliminary study of the structure and permeability of the embryonic coat was carried out in control embryos. The analysis by electron microscopy revealed that its structure is formed by four embryonic envelopes composed of multiple layers while the assay with a fluorescent probe revealed that the embryonic coat increases its permeability during development. STM caused significant histopathological alterations in the hepatopancreas and gills of larvae. This study showed that although the embryos of M. borellii could be protected by the embryonic coat, the larvae are very vulnerable to the STM toxicity. So, it is necessary to continue evaluating the effects of these new pesticides on non-target organisms, such as aquacultured species, to help predict their ecotoxicological risks derived from the increasing agricultural activity developed worldwide. Instituto de Limnología "Dr. Raúl A. Ringuelet" Articulo Articulo http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
spellingShingle Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
Lavarías, Sabrina María Luisa
Arrighetti, Florencia
Landro, Sonia Maribel
Delevati Colpo, Karine
Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
topic_facet Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
description The aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live larvae were fixed for histological analysis. In addition, ovigerous females were exposed to a sublethal concentration of STM (1.7 mg/L) for 96 h to evaluate the activity of the enzymes catalase, glutathione-S-transferase, and superoxide dismutase as well as the lipoperoxidation (LPO) and protein oxidation levels in embryos. The larvae showed a high sensitivity to STM evidenced by the LC₅₀-96 h value (0.011 mg/L). On the contrary, the embryos were highly resistant to STM exposure, and no lethal effect was observed in the treatments with high concentrations of this insecticide (LC₅₀-96 h > 150 mg/L). Among all the biochemical parameters evaluated in the embryos exposed to STM, only LPO showed a significant increase compared to controls. This was probably due to a restricted entry of the insecticide through the embryonic coat. Thus, a preliminary study of the structure and permeability of the embryonic coat was carried out in control embryos. The analysis by electron microscopy revealed that its structure is formed by four embryonic envelopes composed of multiple layers while the assay with a fluorescent probe revealed that the embryonic coat increases its permeability during development. STM caused significant histopathological alterations in the hepatopancreas and gills of larvae. This study showed that although the embryos of M. borellii could be protected by the embryonic coat, the larvae are very vulnerable to the STM toxicity. So, it is necessary to continue evaluating the effects of these new pesticides on non-target organisms, such as aquacultured species, to help predict their ecotoxicological risks derived from the increasing agricultural activity developed worldwide.
format Articulo
Articulo
author Lavarías, Sabrina María Luisa
Arrighetti, Florencia
Landro, Sonia Maribel
Delevati Colpo, Karine
author_facet Lavarías, Sabrina María Luisa
Arrighetti, Florencia
Landro, Sonia Maribel
Delevati Colpo, Karine
author_sort Lavarías, Sabrina María Luisa
title Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_short Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_full Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_fullStr Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_full_unstemmed Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_sort sensitivity of embryos and larvae of the freshwater prawn macrobrachium borellii to the latest generation pesticide spirotetramat
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/155695
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AT arrighettiflorencia sensitivityofembryosandlarvaeofthefreshwaterprawnmacrobrachiumborelliitothelatestgenerationpesticidespirotetramat
AT landrosoniamaribel sensitivityofembryosandlarvaeofthefreshwaterprawnmacrobrachiumborelliitothelatestgenerationpesticidespirotetramat
AT delevaticolpokarine sensitivityofembryosandlarvaeofthefreshwaterprawnmacrobrachiumborelliitothelatestgenerationpesticidespirotetramat
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