Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex

The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal...

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Autores principales: Restrepo Guerrero, Andrés Gonzalo, Goitía, Helen, Naso, Luciana Gissella, Rey, Marilin, Gonzalez, Pablo Javier, Ferrer, Evelina Gloria, Williams, Patricia Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/155666
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-155666
record_format dspace
spelling I19-R120-10915-1556662023-07-31T20:08:37Z http://sedici.unlp.edu.ar/handle/10915/155666 Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex Restrepo Guerrero, Andrés Gonzalo Goitía, Helen Naso, Luciana Gissella Rey, Marilin Gonzalez, Pablo Javier Ferrer, Evelina Gloria Williams, Patricia Ana María 2022-01 2023-07-31T18:43:58Z en Ciencias Exactas Química glycosylated flavonoid oxidovanadium(IV) complex antitumoral antioxidant The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order). Centro de Química Inorgánica Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
spellingShingle Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
Restrepo Guerrero, Andrés Gonzalo
Goitía, Helen
Naso, Luciana Gissella
Rey, Marilin
Gonzalez, Pablo Javier
Ferrer, Evelina Gloria
Williams, Patricia Ana María
Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
topic_facet Ciencias Exactas
Química
glycosylated flavonoid
oxidovanadium(IV) complex
antitumoral
antioxidant
description The complex of oxidovanadium(IV) with naringin (Narg) [VO(Narg)₂] 8H₂O (VONarg) was prepared according to the literature improving the synthetic procedure and physicochemical characterization. In addition, biological activities (cytotoxic, antioxidant, and BSA interaction) were determined. The metal coordinated through the 5-hydroxy and 4-carbonyl groups of rings A and C of naringin, respectively. The antioxidant activity of VONarg, determined in vitro, was higher than those of the flavonoid against superoxide and peroxyl reactive oxygen species (ROS) and DPPH radical. The cytotoxic properties were determined by a MTT assay on adenocarcinoma human alveolar basal epithelial cells (A549). VONarg exerted a 20% decrease in cancer cells viability at 24 h incubation, while naringin and oxidovanadium(IV) cation did not show cytotoxicity. Measurements with the normal HEK293 cell line showed that the inhibitory action of the complex is selective. VONarg generated intracellular reactive oxygen species (ROS), depletion of reduced glutathione and depolarization of mitochondrial membrane potential, typical for apoptotic pathway, producing cell death by oxidative stress mechanism. Moreover, naringin interacted with bovine serum albumin (BSA) through hydrophobic interactions in a spontaneous process, and VONarg showed greater affinity for the protein but can still be transported and delivered by it (Kₐ 10⁴ L⋅mol⁻¹ order).
format Articulo
Articulo
author Restrepo Guerrero, Andrés Gonzalo
Goitía, Helen
Naso, Luciana Gissella
Rey, Marilin
Gonzalez, Pablo Javier
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author_facet Restrepo Guerrero, Andrés Gonzalo
Goitía, Helen
Naso, Luciana Gissella
Rey, Marilin
Gonzalez, Pablo Javier
Ferrer, Evelina Gloria
Williams, Patricia Ana María
author_sort Restrepo Guerrero, Andrés Gonzalo
title Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_short Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_full Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_fullStr Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_full_unstemmed Antioxidant and Anticancer Activities and Protein Interaction of the Oxidovanadium(IV) Naringin Complex
title_sort antioxidant and anticancer activities and protein interaction of the oxidovanadium(iv) naringin complex
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/155666
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AT goitiahelen antioxidantandanticanceractivitiesandproteininteractionoftheoxidovanadiumivnaringincomplex
AT nasolucianagissella antioxidantandanticanceractivitiesandproteininteractionoftheoxidovanadiumivnaringincomplex
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AT gonzalezpablojavier antioxidantandanticanceractivitiesandproteininteractionoftheoxidovanadiumivnaringincomplex
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