PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding

The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into...

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Detalles Bibliográficos
Autores principales: Montero Jimenez, Marjorie, Amante, Francisco L., Fenoy, Gonzalo Eduardo, Scotto, Juliana, Azzaroni, Omar, Marmisollé, Waldemar Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/154186
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-154186
record_format dspace
spelling I19-R120-10915-1541862023-11-28T20:07:26Z http://sedici.unlp.edu.ar/handle/10915/154186 PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding Montero Jimenez, Marjorie Amante, Francisco L. Fenoy, Gonzalo Eduardo Scotto, Juliana Azzaroni, Omar Marmisollé, Waldemar Alejandro 2023 2023-06-09T16:34:25Z en Química Organic electrochemical transistors Conducting polymers Protein binding PEDOT The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
spellingShingle Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
Montero Jimenez, Marjorie
Amante, Francisco L.
Fenoy, Gonzalo Eduardo
Scotto, Juliana
Azzaroni, Omar
Marmisollé, Waldemar Alejandro
PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
topic_facet Química
Organic electrochemical transistors
Conducting polymers
Protein binding
PEDOT
description The fabrication of efficient organic electrochemical transistors (OECTs)-based biosensors requires the design of biocompatible interfaces for the immobilization of biorecognition elements, as well as the development of robust channel materials to enable the transduction of the biochemical event into a reliable electrical signal. In this work, PEDOT-polyamine blends are shown as versatile organic films that can act as both highly conducting channels of the transistors and non-denaturing platforms for the construction of the biomolecular architectures that operate as sensing surfaces. To achieve this goal, we synthesized and characterized films of PEDOT and polyallylamine hydrochloride (PAH) and employed them as conducting channels in the construction of OECTs. Next, we studied the response of the obtained devices to protein adsorption, using glucose oxidase (GOx) as a model system, through two different strategies: The direct electrostatic adsorption of GOx on the PEDOT-PAH film and the specific recognition of the protein by a lectin attached to the surface. Firstly, we used surface plasmon resonance to monitor the adsorption of the proteins and the stability of the assemblies on PEDOT-PAH films. Then, we monitored the same processes with the OECT showing the capability of the device to perform the detection of the protein binding process in real time. In addition, the sensing mechanisms enabling the monitoring of the adsorption process with the OECTs for the two strategies are discussed.
format Articulo
Articulo
author Montero Jimenez, Marjorie
Amante, Francisco L.
Fenoy, Gonzalo Eduardo
Scotto, Juliana
Azzaroni, Omar
Marmisollé, Waldemar Alejandro
author_facet Montero Jimenez, Marjorie
Amante, Francisco L.
Fenoy, Gonzalo Eduardo
Scotto, Juliana
Azzaroni, Omar
Marmisollé, Waldemar Alejandro
author_sort Montero Jimenez, Marjorie
title PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_short PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_full PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_fullStr PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_full_unstemmed PEDOT-polyamine-based organic electrochemical transistors for monitoring protein binding
title_sort pedot-polyamine-based organic electrochemical transistors for monitoring protein binding
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/154186
work_keys_str_mv AT monterojimenezmarjorie pedotpolyaminebasedorganicelectrochemicaltransistorsformonitoringproteinbinding
AT amantefranciscol pedotpolyaminebasedorganicelectrochemicaltransistorsformonitoringproteinbinding
AT fenoygonzaloeduardo pedotpolyaminebasedorganicelectrochemicaltransistorsformonitoringproteinbinding
AT scottojuliana pedotpolyaminebasedorganicelectrochemicaltransistorsformonitoringproteinbinding
AT azzaroniomar pedotpolyaminebasedorganicelectrochemicaltransistorsformonitoringproteinbinding
AT marmisollewaldemaralejandro pedotpolyaminebasedorganicelectrochemicaltransistorsformonitoringproteinbinding
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