Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells

Bisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phospha...

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Autores principales: Molinuevo, María Silvina, Bruzzone, Liliana, Cortizo, Ana María
Formato: Articulo
Lenguaje:Español
Publicado: 2007
Materias:
Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153967
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153967
record_format dspace
spelling I19-R120-10915-1539672023-06-06T04:06:55Z http://sedici.unlp.edu.ar/handle/10915/153967 issn:0014-2999 Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells Molinuevo, María Silvina Bruzzone, Liliana Cortizo, Ana María 2007 2023-06-05T18:49:28Z es Biología Spheroid Bisphosphonates Phosphatase Actin fibers Osteosarcoma Bisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phosphatase of UMR106 rat osteosarcoma cells. In this study, we evaluated the effects of alendronate on the migration of UMR106 osteosarcoma using a model of multicellular cell spheroids, as well as the alendronate effect on neutral phosphatases. Alendronate significantly inhibited the migration of osteoblasts in a dose-dependent manner (10−6–10−4 M). This effect was also dependent on calcium availability. The spheroid morphology and distribution of actin fibers were also affected by alendronate treatment. Alendronate dose-dependently inhibited neutral phosphatase activity in cell-free osteoblastic extracts as well as in osteoblasts in culture. Our results show that alendronate inhibits cell migration through mechanisms dependent on calcium, and that seem to involve inhibition of phosphotyrosine-neutral-phosphatases and disassembly of actin stress fibers. Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 28-33
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
spellingShingle Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
Molinuevo, María Silvina
Bruzzone, Liliana
Cortizo, Ana María
Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
topic_facet Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
description Bisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phosphatase of UMR106 rat osteosarcoma cells. In this study, we evaluated the effects of alendronate on the migration of UMR106 osteosarcoma using a model of multicellular cell spheroids, as well as the alendronate effect on neutral phosphatases. Alendronate significantly inhibited the migration of osteoblasts in a dose-dependent manner (10−6–10−4 M). This effect was also dependent on calcium availability. The spheroid morphology and distribution of actin fibers were also affected by alendronate treatment. Alendronate dose-dependently inhibited neutral phosphatase activity in cell-free osteoblastic extracts as well as in osteoblasts in culture. Our results show that alendronate inhibits cell migration through mechanisms dependent on calcium, and that seem to involve inhibition of phosphotyrosine-neutral-phosphatases and disassembly of actin stress fibers.
format Articulo
Articulo
author Molinuevo, María Silvina
Bruzzone, Liliana
Cortizo, Ana María
author_facet Molinuevo, María Silvina
Bruzzone, Liliana
Cortizo, Ana María
author_sort Molinuevo, María Silvina
title Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_short Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_full Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_fullStr Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_full_unstemmed Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_sort alendronate induces anti-migratory effects and inhibition of neutral phosphatases in umr106 osteosarcoma cells
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/153967
work_keys_str_mv AT molinuevomariasilvina alendronateinducesantimigratoryeffectsandinhibitionofneutralphosphatasesinumr106osteosarcomacells
AT bruzzoneliliana alendronateinducesantimigratoryeffectsandinhibitionofneutralphosphatasesinumr106osteosarcomacells
AT cortizoanamaria alendronateinducesantimigratoryeffectsandinhibitionofneutralphosphatasesinumr106osteosarcomacells
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