Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells

Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescenc...

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Detalles Bibliográficos
Autores principales: Mercer, Natalia, Ahmed, Hafiz, Etcheverry, Susana Beatriz, Vasta, Gerardo R., Cortizo, Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Biología
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153966
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153966
record_format dspace
spelling I19-R120-10915-1539662023-06-06T04:06:57Z http://sedici.unlp.edu.ar/handle/10915/153966 issn:1573-4919 Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells Mercer, Natalia Ahmed, Hafiz Etcheverry, Susana Beatriz Vasta, Gerardo R. Cortizo, Ana María 2007-07-28 2023-06-05T18:43:15Z en Biología Advanced glycation end products RAGE Galectin-3 Osteoblasts Apoptosis AGE-receptors Regulation Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients. Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 87-94
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
spellingShingle Biología
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
Mercer, Natalia
Ahmed, Hafiz
Etcheverry, Susana Beatriz
Vasta, Gerardo R.
Cortizo, Ana María
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
topic_facet Biología
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
description Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
format Articulo
Articulo
author Mercer, Natalia
Ahmed, Hafiz
Etcheverry, Susana Beatriz
Vasta, Gerardo R.
Cortizo, Ana María
author_facet Mercer, Natalia
Ahmed, Hafiz
Etcheverry, Susana Beatriz
Vasta, Gerardo R.
Cortizo, Ana María
author_sort Mercer, Natalia
title Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_short Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_full Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_fullStr Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_full_unstemmed Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_sort regulation of advanced glycation end product (age) receptors and apoptosis by ages in osteoblast-like cells
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/153966
work_keys_str_mv AT mercernatalia regulationofadvancedglycationendproductagereceptorsandapoptosisbyagesinosteoblastlikecells
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AT etcheverrysusanabeatriz regulationofadvancedglycationendproductagereceptorsandapoptosisbyagesinosteoblastlikecells
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