First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina

Sarcocystis is a genus of intracellular parasitic protozoa that infects various species of mammals, birds, and reptiles worldwide. At least 46 Sarcocystis species naturally infect rodents as intermediate hosts producing tissue cysts. This study aimed to provide the first report and molecular charact...

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Autores principales: Canova, Victoria, Helman, María Elisa, Robles, María del Rosario, Abba, Agustín Manuel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Biología
Sarcocystis spp.
Plains viscacha
PCR
Argentina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153403
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153403
record_format dspace
spelling I19-R120-10915-1534032023-05-23T04:07:16Z http://sedici.unlp.edu.ar/handle/10915/153403 issn:2213-2244 First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina Canova, Victoria Helman, María Elisa Robles, María del Rosario Abba, Agustín Manuel 2023 2023-05-22T16:35:54Z en Biología Sarcocystis spp. Plains viscacha PCR Argentina Sarcocystis is a genus of intracellular parasitic protozoa that infects various species of mammals, birds, and reptiles worldwide. At least 46 Sarcocystis species naturally infect rodents as intermediate hosts producing tissue cysts. This study aimed to provide the first report and molecular characterisation of Sarcocystis spp. in muscles from plains viscacha (Lagostomus maximus) in Argentina. Muscle samples of 53 plains viscachas from three provinces of Argentina were processed by homogenisation and optical microscopy to detect tissue cysts. Positive samples were analysed by PCR-sequencing, using the following markers: 18S rRNA, ITS1, and coxI. The 18S rRNA and coxI consensus sequences were aligned with other sequences from Sarcocystis spp., and phylogenetic trees were constructed. Of all animals processed, 13.2% (7/53) harboured Sarcocystis sp. cysts. 18S rRNA consensus sequences were obtained from four muscle samples and one individual cyst, and they showed 99.88–100% similarity, except for the cyst sequence, which showed 97.11% homology. Similarities of only 96–97% were recorded in the 18S rRNA fragment with other Sarcocystis spp. whose sequences are available in the GenBank. The five coxI fragment sequences obtained were 100% identical and showed an identity of 99.41–99.48% with S. canis. For ITS1 only short and low-quality sequences were obtained. In the phylogenetic trees, all the sequences from plains viscachas were positioned together in a branch separated from other Sarcocystis spp. These results could be related to new Sarcocystis spp. producing sarcocysts in plains viscachas. Besides, comprehensive cyst morphological analysis using TEM from the new Sarcocystis species will allow a description of the cyst wall ultrastructure. In this sense, further studies are needed to deepen these findings and elucidate other potential intermediate and possible definitive hosts. Centro de Estudios Parasitológicos y de Vectores Laboratorio de Inmunoparasitología Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 180-186
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Sarcocystis spp.
Plains viscacha
PCR
Argentina
spellingShingle Biología
Sarcocystis spp.
Plains viscacha
PCR
Argentina
Canova, Victoria
Helman, María Elisa
Robles, María del Rosario
Abba, Agustín Manuel
First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina
topic_facet Biología
Sarcocystis spp.
Plains viscacha
PCR
Argentina
description Sarcocystis is a genus of intracellular parasitic protozoa that infects various species of mammals, birds, and reptiles worldwide. At least 46 Sarcocystis species naturally infect rodents as intermediate hosts producing tissue cysts. This study aimed to provide the first report and molecular characterisation of Sarcocystis spp. in muscles from plains viscacha (Lagostomus maximus) in Argentina. Muscle samples of 53 plains viscachas from three provinces of Argentina were processed by homogenisation and optical microscopy to detect tissue cysts. Positive samples were analysed by PCR-sequencing, using the following markers: 18S rRNA, ITS1, and coxI. The 18S rRNA and coxI consensus sequences were aligned with other sequences from Sarcocystis spp., and phylogenetic trees were constructed. Of all animals processed, 13.2% (7/53) harboured Sarcocystis sp. cysts. 18S rRNA consensus sequences were obtained from four muscle samples and one individual cyst, and they showed 99.88–100% similarity, except for the cyst sequence, which showed 97.11% homology. Similarities of only 96–97% were recorded in the 18S rRNA fragment with other Sarcocystis spp. whose sequences are available in the GenBank. The five coxI fragment sequences obtained were 100% identical and showed an identity of 99.41–99.48% with S. canis. For ITS1 only short and low-quality sequences were obtained. In the phylogenetic trees, all the sequences from plains viscachas were positioned together in a branch separated from other Sarcocystis spp. These results could be related to new Sarcocystis spp. producing sarcocysts in plains viscachas. Besides, comprehensive cyst morphological analysis using TEM from the new Sarcocystis species will allow a description of the cyst wall ultrastructure. In this sense, further studies are needed to deepen these findings and elucidate other potential intermediate and possible definitive hosts.
format Articulo
Articulo
author Canova, Victoria
Helman, María Elisa
Robles, María del Rosario
Abba, Agustín Manuel
author_facet Canova, Victoria
Helman, María Elisa
Robles, María del Rosario
Abba, Agustín Manuel
author_sort Canova, Victoria
title First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina
title_short First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina
title_full First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina
title_fullStr First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina
title_full_unstemmed First report of Sarcocystis spp. (Apicomplexa, Sarcocystidae) in Lagostomus maximus (Desmarest, 1917) (Rodentia, Chinchillidae) in Argentina
title_sort first report of sarcocystis spp. (apicomplexa, sarcocystidae) in lagostomus maximus (desmarest, 1917) (rodentia, chinchillidae) in argentina
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/153403
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AT helmanmariaelisa firstreportofsarcocystissppapicomplexasarcocystidaeinlagostomusmaximusdesmarest1917rodentiachinchillidaeinargentina
AT roblesmariadelrosario firstreportofsarcocystissppapicomplexasarcocystidaeinlagostomusmaximusdesmarest1917rodentiachinchillidaeinargentina
AT abbaagustinmanuel firstreportofsarcocystissppapicomplexasarcocystidaeinlagostomusmaximusdesmarest1917rodentiachinchillidaeinargentina
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