Silk coatings on PLGA and alginate microspheres for protein delivery

Bombyx mori silk fibroin self-assembles on surfaces to form ultrathin nanoscale coatings based on our prior studies using layer-bylayer deposition techniques driven by hydrophobic interactions between silk fibroin protein molecules. In the present study, poly(lacticco- glycolic acid) (PLGA) and algi...

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Autores principales: Wang, Xiaoqin, Wenk, Esther, Hu, Xiao, Castro, Guillermo Raúl, Meinel, Lorenz, Wang, Xianyan, Li, Chunmei, Merkle, Hans, Kaplan, David L.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Bioquímica
Silk
Fibroin
Alginate
Polylactic acid
Polyglycolic acid
Controlled release
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153291
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153291
record_format dspace
spelling I19-R120-10915-1532912023-05-20T04:05:58Z http://sedici.unlp.edu.ar/handle/10915/153291 issn:1878-5905 issn:0142-9612 Silk coatings on PLGA and alginate microspheres for protein delivery Wang, Xiaoqin Wenk, Esther Hu, Xiao Castro, Guillermo Raúl Meinel, Lorenz Wang, Xianyan Li, Chunmei Merkle, Hans Kaplan, David L. 2007 2023-05-19T16:28:41Z en Bioquímica Silk Fibroin Alginate Polylactic acid Polyglycolic acid Controlled release Bombyx mori silk fibroin self-assembles on surfaces to form ultrathin nanoscale coatings based on our prior studies using layer-bylayer deposition techniques driven by hydrophobic interactions between silk fibroin protein molecules. In the present study, poly(lacticco- glycolic acid) (PLGA) and alginate microspheres were used as substrates and coated with silk fibroin. The coatings were visualized by confocal laser scanning microscopy using fluorescein-labeled silk fibroin. On PLGA microspheres, the coating was ∼1 μm and discontinuous, reflecting the porous surface of these microspheres determined by SEM. In contrast, on alginate microspheres the coating was ∼10 μm thick and continuous. The silk fibroin penetrated into the alginate gel matrix. The silk coating on the PLGA microspheres delayed PLGA degradation. The silk coating on the alginate microspheres survived ethylenediamine tetraacetic acid (EDTA) treatment used to remove the Ca2+-cross-links in the alginate gels to solubilize the alginate. This suggests that alginate microspheres can be used as templates to form silk microcapsules. Horseradish peroxidase (HRP) and tetramethylrhodamine-conjugated bovine serum albumin (Rh-BSA) as model protein drugs were encapsulated in the PLGA and alginate microspheres with and without the silk fibroin coatings. Drug release was significantly retarded by the silk coatings when compared to uncoated microsphere controls, and was retarded further by methanol-treated silk coating when compared to silk water-based coatings on alginate microspheres. Silk coatings on PLGA and alginate microspheres provide mechanically stable shells as well as a diffusion barrier to the encapsulated protein drugs. This coating technique has potential for biosensor and drug delivery applications due to the aqueous process employed, the ability to control coating thickness and crystalline content, and the biocompatibility of the silk fibroin protein used in the process. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 4161-4169
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Silk
Fibroin
Alginate
Polylactic acid
Polyglycolic acid
Controlled release
spellingShingle Bioquímica
Silk
Fibroin
Alginate
Polylactic acid
Polyglycolic acid
Controlled release
Wang, Xiaoqin
Wenk, Esther
Hu, Xiao
Castro, Guillermo Raúl
Meinel, Lorenz
Wang, Xianyan
Li, Chunmei
Merkle, Hans
Kaplan, David L.
Silk coatings on PLGA and alginate microspheres for protein delivery
topic_facet Bioquímica
Silk
Fibroin
Alginate
Polylactic acid
Polyglycolic acid
Controlled release
description Bombyx mori silk fibroin self-assembles on surfaces to form ultrathin nanoscale coatings based on our prior studies using layer-bylayer deposition techniques driven by hydrophobic interactions between silk fibroin protein molecules. In the present study, poly(lacticco- glycolic acid) (PLGA) and alginate microspheres were used as substrates and coated with silk fibroin. The coatings were visualized by confocal laser scanning microscopy using fluorescein-labeled silk fibroin. On PLGA microspheres, the coating was ∼1 μm and discontinuous, reflecting the porous surface of these microspheres determined by SEM. In contrast, on alginate microspheres the coating was ∼10 μm thick and continuous. The silk fibroin penetrated into the alginate gel matrix. The silk coating on the PLGA microspheres delayed PLGA degradation. The silk coating on the alginate microspheres survived ethylenediamine tetraacetic acid (EDTA) treatment used to remove the Ca2+-cross-links in the alginate gels to solubilize the alginate. This suggests that alginate microspheres can be used as templates to form silk microcapsules. Horseradish peroxidase (HRP) and tetramethylrhodamine-conjugated bovine serum albumin (Rh-BSA) as model protein drugs were encapsulated in the PLGA and alginate microspheres with and without the silk fibroin coatings. Drug release was significantly retarded by the silk coatings when compared to uncoated microsphere controls, and was retarded further by methanol-treated silk coating when compared to silk water-based coatings on alginate microspheres. Silk coatings on PLGA and alginate microspheres provide mechanically stable shells as well as a diffusion barrier to the encapsulated protein drugs. This coating technique has potential for biosensor and drug delivery applications due to the aqueous process employed, the ability to control coating thickness and crystalline content, and the biocompatibility of the silk fibroin protein used in the process.
format Articulo
Articulo
author Wang, Xiaoqin
Wenk, Esther
Hu, Xiao
Castro, Guillermo Raúl
Meinel, Lorenz
Wang, Xianyan
Li, Chunmei
Merkle, Hans
Kaplan, David L.
author_facet Wang, Xiaoqin
Wenk, Esther
Hu, Xiao
Castro, Guillermo Raúl
Meinel, Lorenz
Wang, Xianyan
Li, Chunmei
Merkle, Hans
Kaplan, David L.
author_sort Wang, Xiaoqin
title Silk coatings on PLGA and alginate microspheres for protein delivery
title_short Silk coatings on PLGA and alginate microspheres for protein delivery
title_full Silk coatings on PLGA and alginate microspheres for protein delivery
title_fullStr Silk coatings on PLGA and alginate microspheres for protein delivery
title_full_unstemmed Silk coatings on PLGA and alginate microspheres for protein delivery
title_sort silk coatings on plga and alginate microspheres for protein delivery
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/153291
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