Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity

Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymer...

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Autores principales: Dini, Cecilia, Islan, Germán Abel, Urraza, Patricio José de, Castro, Guillermo Raúl
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153287
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153287
record_format dspace
spelling I19-R120-10915-1532872023-05-20T04:06:05Z http://sedici.unlp.edu.ar/handle/10915/153287 issn:1616-5195 Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity Dini, Cecilia Islan, Germán Abel Urraza, Patricio José de Castro, Guillermo Raúl 2012 2023-05-19T16:06:21Z en Biología Biocontrol Biological applications of polymers Biopolymers Microencapsulation Phages Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10³ active phages after 30 min exposure at pH¼1.6, and protects phage from pepsin activity (4.2mgmL⁻¹). Non-encapsulated phages are fully inactivated at pH¼1.6 or with pepsin (0.5mgmL⁻¹) after 10 min. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 1200–1208
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
spellingShingle Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
Dini, Cecilia
Islan, Germán Abel
Urraza, Patricio José de
Castro, Guillermo Raúl
Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
topic_facet Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
description Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10³ active phages after 30 min exposure at pH¼1.6, and protects phage from pepsin activity (4.2mgmL⁻¹). Non-encapsulated phages are fully inactivated at pH¼1.6 or with pepsin (0.5mgmL⁻¹) after 10 min.
format Articulo
Articulo
author Dini, Cecilia
Islan, Germán Abel
Urraza, Patricio José de
Castro, Guillermo Raúl
author_facet Dini, Cecilia
Islan, Germán Abel
Urraza, Patricio José de
Castro, Guillermo Raúl
author_sort Dini, Cecilia
title Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_short Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_full Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_fullStr Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_full_unstemmed Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_sort novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/153287
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