Emulsan quantitation by Nile red quenching fluorescence assay
A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emuls...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2005
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/153222 |
| Aporte de: |
| Sumario: | A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹. |
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