Emulsan quantitation by Nile red quenching fluorescence assay
A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emuls...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2005
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/153222 |
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I19-R120-10915-153222 |
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I19-R120-10915-1532222023-05-19T04:07:11Z http://sedici.unlp.edu.ar/handle/10915/153222 issn:0175-7598 issn:1432-0614 Emulsan quantitation by Nile red quenching fluorescence assay Castro, Guillermo Raúl Larson, Bridget K. Panilaitis, Bruce Kaplan, David L. 2005 2023-05-18T17:13:29Z en Biología Emulsan Polyhydroxyalkanoates Emulsan concentration Turbidimetric assay Polyhydroxyalkanoic acid A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 767–770 |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Emulsan Polyhydroxyalkanoates Emulsan concentration Turbidimetric assay Polyhydroxyalkanoic acid |
| spellingShingle |
Biología Emulsan Polyhydroxyalkanoates Emulsan concentration Turbidimetric assay Polyhydroxyalkanoic acid Castro, Guillermo Raúl Larson, Bridget K. Panilaitis, Bruce Kaplan, David L. Emulsan quantitation by Nile red quenching fluorescence assay |
| topic_facet |
Biología Emulsan Polyhydroxyalkanoates Emulsan concentration Turbidimetric assay Polyhydroxyalkanoic acid |
| description |
A Nile red fluorescent technique to quantify 20– 200 μg ml⁻¹ of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm⁻¹ M⁻¹. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG⁻¹. |
| format |
Articulo Articulo |
| author |
Castro, Guillermo Raúl Larson, Bridget K. Panilaitis, Bruce Kaplan, David L. |
| author_facet |
Castro, Guillermo Raúl Larson, Bridget K. Panilaitis, Bruce Kaplan, David L. |
| author_sort |
Castro, Guillermo Raúl |
| title |
Emulsan quantitation by Nile red quenching fluorescence assay |
| title_short |
Emulsan quantitation by Nile red quenching fluorescence assay |
| title_full |
Emulsan quantitation by Nile red quenching fluorescence assay |
| title_fullStr |
Emulsan quantitation by Nile red quenching fluorescence assay |
| title_full_unstemmed |
Emulsan quantitation by Nile red quenching fluorescence assay |
| title_sort |
emulsan quantitation by nile red quenching fluorescence assay |
| publishDate |
2005 |
| url |
http://sedici.unlp.edu.ar/handle/10915/153222 |
| work_keys_str_mv |
AT castroguillermoraul emulsanquantitationbynileredquenchingfluorescenceassay AT larsonbridgetk emulsanquantitationbynileredquenchingfluorescenceassay AT panilaitisbruce emulsanquantitationbynileredquenchingfluorescenceassay AT kaplandavidl emulsanquantitationbynileredquenchingfluorescenceassay |
| _version_ |
1766370258261639168 |