Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment

A keratinase isolated from Paecilomyces lilacinus (LPS #876) was tested against proteins present in the skin but the high enzyme activity was detected on collagen. Keratinase was physically immobilized onto PVA–pectin cryogels and enzyme release was 20.8 ± 2.1%, 63.8 ± 0.2%, 41.5 ± 3.5% and 26.0 ± 3...

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Autores principales: Martínez, Yanina, Cavello, Ivana Alejandra, Hours, Roque Alberto, Cavalitto, Sebastián Fernando, Castro, Guillermo R.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Bioquímica
PVA cryogels
Keratinase
Enrofloxacin
Controlled release
Wound healing
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153219
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153219
record_format dspace
spelling I19-R120-10915-1532192023-05-19T04:07:15Z http://sedici.unlp.edu.ar/handle/10915/153219 issn:1873-2976 issn:1873-2976 Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment Martínez, Yanina Cavello, Ivana Alejandra Hours, Roque Alberto Cavalitto, Sebastián Fernando Castro, Guillermo R. 2013 2023-05-18T16:58:07Z en Bioquímica PVA cryogels Keratinase Enrofloxacin Controlled release Wound healing A keratinase isolated from Paecilomyces lilacinus (LPS #876) was tested against proteins present in the skin but the high enzyme activity was detected on collagen. Keratinase was physically immobilized onto PVA–pectin cryogels and enzyme release was 20.8 ± 2.1%, 63.8 ± 0.2%, 41.5 ± 3.5% and 26.0 ± 3.5% in cryogels containing pectins with esterification degrees (DE) 33.0%, 55.0%, 62.7% and 71.7% respectively at 37 ºC after 3 h incubation. In presence of 0.75 M NaCl, the percentage of enzyme release changed to: 57.5 ± 1.5, 65.8 ± 3.8, 57.3 ± 0.2 and 34.0 ± 4.0 for the four pectins respectively. In-vitro studies of enrofloxacin release from PVA–pectin cryogels at pH close to the human skin (pH = 5.5) showed 15.0% free antibiotic following first order kinetic at 37 ºC after 5 h incubation. However, in the presence of keratinase only 6.9% of enrofloxacin was released under the same experimental conditions. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 280-284
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
PVA cryogels
Keratinase
Enrofloxacin
Controlled release
Wound healing
spellingShingle Bioquímica
PVA cryogels
Keratinase
Enrofloxacin
Controlled release
Wound healing
Martínez, Yanina
Cavello, Ivana Alejandra
Hours, Roque Alberto
Cavalitto, Sebastián Fernando
Castro, Guillermo R.
Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment
topic_facet Bioquímica
PVA cryogels
Keratinase
Enrofloxacin
Controlled release
Wound healing
description A keratinase isolated from Paecilomyces lilacinus (LPS #876) was tested against proteins present in the skin but the high enzyme activity was detected on collagen. Keratinase was physically immobilized onto PVA–pectin cryogels and enzyme release was 20.8 ± 2.1%, 63.8 ± 0.2%, 41.5 ± 3.5% and 26.0 ± 3.5% in cryogels containing pectins with esterification degrees (DE) 33.0%, 55.0%, 62.7% and 71.7% respectively at 37 ºC after 3 h incubation. In presence of 0.75 M NaCl, the percentage of enzyme release changed to: 57.5 ± 1.5, 65.8 ± 3.8, 57.3 ± 0.2 and 34.0 ± 4.0 for the four pectins respectively. In-vitro studies of enrofloxacin release from PVA–pectin cryogels at pH close to the human skin (pH = 5.5) showed 15.0% free antibiotic following first order kinetic at 37 ºC after 5 h incubation. However, in the presence of keratinase only 6.9% of enrofloxacin was released under the same experimental conditions.
format Articulo
Articulo
author Martínez, Yanina
Cavello, Ivana Alejandra
Hours, Roque Alberto
Cavalitto, Sebastián Fernando
Castro, Guillermo R.
author_facet Martínez, Yanina
Cavello, Ivana Alejandra
Hours, Roque Alberto
Cavalitto, Sebastián Fernando
Castro, Guillermo R.
author_sort Martínez, Yanina
title Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment
title_short Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment
title_full Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment
title_fullStr Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment
title_full_unstemmed Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment
title_sort immobilized keratinase and enrofloxacin loaded on pectin pva cryogel patches for antimicrobial treatment
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/153219
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