Properties and applications of phytepsins from thistle flowers

Aqueous extracts of thistle flowers from the genus Cynara—Cardueae tribe Cass. (Cynareae Less.), Asteraceae Dumortier—are traditionally used in the Mediterranean region for production of artisanal cheeses. This is because of the presence of aspartic proteases (APs) with the ability to coagulate mil...

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Detalles Bibliográficos
Autores principales:	Vairo Cavalli, Sandra Elizabeth, Lufrano, Daniela, Colombo, María Laura, Priolo de Lufrano, Nora Silvia
Formato:	Articulo Revision
Lenguaje:	Inglés
Publicado:	2013
Materias:	Biología Plant aspartic proteases Thistle flowers Asteraceae Phytepsins
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/153188
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-153188
record_format	dspace
spelling	I19-R120-10915-1531882023-05-19T04:07:00Z http://sedici.unlp.edu.ar/handle/10915/153188 issn:0031-9422 Properties and applications of phytepsins from thistle flowers Vairo Cavalli, Sandra Elizabeth Lufrano, Daniela Colombo, María Laura Priolo de Lufrano, Nora Silvia 2013 2023-05-18T15:15:04Z en Biología Plant aspartic proteases Thistle flowers Asteraceae Phytepsins Aqueous extracts of thistle flowers from the genus Cynara—Cardueae tribe Cass. (Cynareae Less.), Asteraceae Dumortier—are traditionally used in the Mediterranean region for production of artisanal cheeses. This is because of the presence of aspartic proteases (APs) with the ability to coagulate milk. Plant APs, collectively known as phytepsins (EC 3.4.23.40), are bilobed endopeptidases present in an ample variety of plant species with activity mainly at acidic pHs, and have two aspartic residues located on each side of a catalytic cleft that are responsible for catalysis. The cleavage of the scissile peptide-bond occurs primarily between residues with large hydrophobic side-chains. Even when aspartylendopeptidase activity in plants is normally present at relatively low levels overall, the flowers of several species of the Cardueae tribe possess APs with extremely high specific activities in certain tissues. For this reason, in the last two decades, APs present in thistle flowers have been the subject of intensive study. Present here is a compilation of work that summarizes the known chemical and biological properties of these proteases, as well as their biomedical and biotechnological applications. Centro de Investigación de Proteínas Vegetales Articulo Revision http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 16-32
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Plant aspartic proteases Thistle flowers Asteraceae Phytepsins
spellingShingle	Biología Plant aspartic proteases Thistle flowers Asteraceae Phytepsins Vairo Cavalli, Sandra Elizabeth Lufrano, Daniela Colombo, María Laura Priolo de Lufrano, Nora Silvia Properties and applications of phytepsins from thistle flowers
topic_facet	Biología Plant aspartic proteases Thistle flowers Asteraceae Phytepsins
description	Aqueous extracts of thistle flowers from the genus Cynara—Cardueae tribe Cass. (Cynareae Less.), Asteraceae Dumortier—are traditionally used in the Mediterranean region for production of artisanal cheeses. This is because of the presence of aspartic proteases (APs) with the ability to coagulate milk. Plant APs, collectively known as phytepsins (EC 3.4.23.40), are bilobed endopeptidases present in an ample variety of plant species with activity mainly at acidic pHs, and have two aspartic residues located on each side of a catalytic cleft that are responsible for catalysis. The cleavage of the scissile peptide-bond occurs primarily between residues with large hydrophobic side-chains. Even when aspartylendopeptidase activity in plants is normally present at relatively low levels overall, the flowers of several species of the Cardueae tribe possess APs with extremely high specific activities in certain tissues. For this reason, in the last two decades, APs present in thistle flowers have been the subject of intensive study. Present here is a compilation of work that summarizes the known chemical and biological properties of these proteases, as well as their biomedical and biotechnological applications.
format	Articulo Revision
author	Vairo Cavalli, Sandra Elizabeth Lufrano, Daniela Colombo, María Laura Priolo de Lufrano, Nora Silvia
author_facet	Vairo Cavalli, Sandra Elizabeth Lufrano, Daniela Colombo, María Laura Priolo de Lufrano, Nora Silvia
author_sort	Vairo Cavalli, Sandra Elizabeth
title	Properties and applications of phytepsins from thistle flowers
title_short	Properties and applications of phytepsins from thistle flowers
title_full	Properties and applications of phytepsins from thistle flowers
title_fullStr	Properties and applications of phytepsins from thistle flowers
title_full_unstemmed	Properties and applications of phytepsins from thistle flowers
title_sort	properties and applications of phytepsins from thistle flowers
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/153188
work_keys_str_mv	AT vairocavallisandraelizabeth propertiesandapplicationsofphytepsinsfromthistleflowers AT lufranodaniela propertiesandapplicationsofphytepsinsfromthistleflowers AT colombomarialaura propertiesandapplicationsofphytepsinsfromthistleflowers AT priolodelufranonorasilvia propertiesandapplicationsofphytepsinsfromthistleflowers
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Properties and applications of phytepsins from thistle flowers

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