Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)

The properties of morrenain b II, a proteinase isolated from the latex of Morrenia brachystephana, were compared with those of morrenain o II, a proteinase obtained from the latex of Morrenia odorata. Both peptidases were purified to homogeneity by acetone precipitation followed by cation exchange c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vairo Cavalli, Sandra Elizabeth, Cortadi, Adriana A., Arribére, María Cecilia, Conforti, Paula Andrea, Caffini, Néstor Oscar, Priolo de Lufrano, Nora Silvia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Química Biología Latex peptidases Morrenain Protein purification Thiol peptidases
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/153185
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-153185
record_format	dspace
spelling	I19-R120-10915-1531852023-05-19T04:07:04Z http://sedici.unlp.edu.ar/handle/10915/153185 issn:1437-4315 Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae) Vairo Cavalli, Sandra Elizabeth Cortadi, Adriana A. Arribére, María Cecilia Conforti, Paula Andrea Caffini, Néstor Oscar Priolo de Lufrano, Nora Silvia 2013 2023-05-18T15:02:13Z en Química Biología Latex peptidases Morrenain Protein purification Thiol peptidases The properties of morrenain b II, a proteinase isolated from the latex of Morrenia brachystephana, were compared with those of morrenain o II, a proteinase obtained from the latex of Morrenia odorata. Both peptidases were purified to homogeneity by acetone precipitation followed by cation exchange chromatography. The enzymes have pi values higher than 9.3 and similar molecular masses (close to 26 kDa) as determined by SDS-PAGE. They display maximum proteolytic activity within an alkaline pH range, and also exhibit esterolytic activity. The N-terminal sequences of morrenain o II and morrenain b II show a high degree of homology between each other and to other cysteine plant proteinases. Centro de Investigación de Proteínas Vegetales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química Biología Latex peptidases Morrenain Protein purification Thiol peptidases
spellingShingle	Química Biología Latex peptidases Morrenain Protein purification Thiol peptidases Vairo Cavalli, Sandra Elizabeth Cortadi, Adriana A. Arribére, María Cecilia Conforti, Paula Andrea Caffini, Néstor Oscar Priolo de Lufrano, Nora Silvia Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)
topic_facet	Química Biología Latex peptidases Morrenain Protein purification Thiol peptidases
description	The properties of morrenain b II, a proteinase isolated from the latex of Morrenia brachystephana, were compared with those of morrenain o II, a proteinase obtained from the latex of Morrenia odorata. Both peptidases were purified to homogeneity by acetone precipitation followed by cation exchange chromatography. The enzymes have pi values higher than 9.3 and similar molecular masses (close to 26 kDa) as determined by SDS-PAGE. They display maximum proteolytic activity within an alkaline pH range, and also exhibit esterolytic activity. The N-terminal sequences of morrenain o II and morrenain b II show a high degree of homology between each other and to other cysteine plant proteinases.
format	Articulo Articulo
author	Vairo Cavalli, Sandra Elizabeth Cortadi, Adriana A. Arribére, María Cecilia Conforti, Paula Andrea Caffini, Néstor Oscar Priolo de Lufrano, Nora Silvia
author_facet	Vairo Cavalli, Sandra Elizabeth Cortadi, Adriana A. Arribére, María Cecilia Conforti, Paula Andrea Caffini, Néstor Oscar Priolo de Lufrano, Nora Silvia
author_sort	Vairo Cavalli, Sandra Elizabeth
title	Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)
title_short	Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)
title_full	Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)
title_fullStr	Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)
title_full_unstemmed	Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)
title_sort	comparison of two cysteine endopeptidases from latices of morrenia brachystephana griseb. and morrenia odorata (hook et arn.) lindley (asclepiadaceae)
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/153185
work_keys_str_mv	AT vairocavallisandraelizabeth comparisonoftwocysteineendopeptidasesfromlaticesofmorreniabrachystephanagrisebandmorreniaodoratahooketarnlindleyasclepiadaceae AT cortadiadrianaa comparisonoftwocysteineendopeptidasesfromlaticesofmorreniabrachystephanagrisebandmorreniaodoratahooketarnlindleyasclepiadaceae AT arriberemariacecilia comparisonoftwocysteineendopeptidasesfromlaticesofmorreniabrachystephanagrisebandmorreniaodoratahooketarnlindleyasclepiadaceae AT confortipaulaandrea comparisonoftwocysteineendopeptidasesfromlaticesofmorreniabrachystephanagrisebandmorreniaodoratahooketarnlindleyasclepiadaceae AT caffininestoroscar comparisonoftwocysteineendopeptidasesfromlaticesofmorreniabrachystephanagrisebandmorreniaodoratahooketarnlindleyasclepiadaceae AT priolodelufranonorasilvia comparisonoftwocysteineendopeptidasesfromlaticesofmorreniabrachystephanagrisebandmorreniaodoratahooketarnlindleyasclepiadaceae
_version_	1766370254253981696

Comparison of Two Cysteine Endopeptidases from Latices of Morrenia brachystephana Griseb. and Morrenia odorata (Hook et Arn.) Lindley (Asclepiadaceae)

Ejemplares similares