Emulsan–alginate beads for protein adsorption

Emulsan–alginate beads were prepared and challenged using bovine serum albumin (BSA) to assess adsorption in comparison to alginate beads. BSA binding to the emulsan–alginate beads was improved over the alginate bead controls and protein adsorption was less sensitive to changes in ionic strength. BS...

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Autores principales: Castro, Guillermo Raúl, Chen, Jingsong, Panilaitis, Bruce;, Kaplan, David L.;
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Bioquímica
Adsorption
Alginate
Emulsan
Hydrogel
Biopolymer
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153005
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153005
record_format dspace
spelling I19-R120-10915-1530052023-05-16T04:06:31Z http://sedici.unlp.edu.ar/handle/10915/153005 issn:0920-5063 issn:1568-5624 Emulsan–alginate beads for protein adsorption Castro, Guillermo Raúl Chen, Jingsong Panilaitis, Bruce; Kaplan, David L.; 2012 2023-05-15T16:45:15Z en Bioquímica Adsorption Alginate Emulsan Hydrogel Biopolymer Emulsan–alginate beads were prepared and challenged using bovine serum albumin (BSA) to assess adsorption in comparison to alginate beads. BSA binding to the emulsan–alginate beads was improved over the alginate bead controls and protein adsorption was less sensitive to changes in ionic strength. BSA adsorption between pH 8.5 and 5.3 in alginate beads was 2–3-times lower compared to the emulsan–alginate beads in the same pH range. BSA adsorption and kinetic constants were at least 2-times higher for the emulsan–alginate beads compared to the alginate controls based on the Langmuir adsorption model. To further explore the utility of these novel emulsan–alginate bead systems, complex cell-free supernatants from some pathogenic microorganisms were exposed to the emulsan–alginate beads and increased protein adsorption was found when compared to controls. These trends were also confirmed with α-hemolysin toxicity studies. The data suggest that the protein-binding capacity of emulsan–alginate beads exceeds alginate controls, attributable to the unique binding features of emulsan. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Adsorption
Alginate
Emulsan
Hydrogel
Biopolymer
spellingShingle Bioquímica
Adsorption
Alginate
Emulsan
Hydrogel
Biopolymer
Castro, Guillermo Raúl
Chen, Jingsong
Panilaitis, Bruce;
Kaplan, David L.;
Emulsan–alginate beads for protein adsorption
topic_facet Bioquímica
Adsorption
Alginate
Emulsan
Hydrogel
Biopolymer
description Emulsan–alginate beads were prepared and challenged using bovine serum albumin (BSA) to assess adsorption in comparison to alginate beads. BSA binding to the emulsan–alginate beads was improved over the alginate bead controls and protein adsorption was less sensitive to changes in ionic strength. BSA adsorption between pH 8.5 and 5.3 in alginate beads was 2–3-times lower compared to the emulsan–alginate beads in the same pH range. BSA adsorption and kinetic constants were at least 2-times higher for the emulsan–alginate beads compared to the alginate controls based on the Langmuir adsorption model. To further explore the utility of these novel emulsan–alginate bead systems, complex cell-free supernatants from some pathogenic microorganisms were exposed to the emulsan–alginate beads and increased protein adsorption was found when compared to controls. These trends were also confirmed with α-hemolysin toxicity studies. The data suggest that the protein-binding capacity of emulsan–alginate beads exceeds alginate controls, attributable to the unique binding features of emulsan.
format Articulo
Articulo
author Castro, Guillermo Raúl
Chen, Jingsong
Panilaitis, Bruce;
Kaplan, David L.;
author_facet Castro, Guillermo Raúl
Chen, Jingsong
Panilaitis, Bruce;
Kaplan, David L.;
author_sort Castro, Guillermo Raúl
title Emulsan–alginate beads for protein adsorption
title_short Emulsan–alginate beads for protein adsorption
title_full Emulsan–alginate beads for protein adsorption
title_fullStr Emulsan–alginate beads for protein adsorption
title_full_unstemmed Emulsan–alginate beads for protein adsorption
title_sort emulsan–alginate beads for protein adsorption
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/153005
work_keys_str_mv AT castroguillermoraul emulsanalginatebeadsforproteinadsorption
AT chenjingsong emulsanalginatebeadsforproteinadsorption
AT panilaitisbruce emulsanalginatebeadsforproteinadsorption
AT kaplandavidl emulsanalginatebeadsforproteinadsorption
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