Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages

The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of...

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Detalles Bibliográficos
Autores principales: Sánchez López, Luna, Ropero de Torres, Noelia, Chico, Belén, Fagali, Natalia, Ríos, Vivian de los, Escudero,María Lorenza, García Alonso, María Cristina, Lozano, Rosa María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2023
Materias:
Química
CoCr
Proteomic analysis
Inflammatory response
Wear
Graphene
Hyaluronic acid
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/152539
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-152539
record_format dspace
spelling I19-R120-10915-1525392023-05-06T04:06:24Z http://sedici.unlp.edu.ar/handle/10915/152539 issn:2075-4701 Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages Sánchez López, Luna Ropero de Torres, Noelia Chico, Belén Fagali, Natalia Ríos, Vivian de los Escudero,María Lorenza García Alonso, María Cristina Lozano, Rosa María 2023 2023-05-05T17:47:08Z en Química CoCr Proteomic analysis Inflammatory response Wear Graphene Hyaluronic acid The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
CoCr
Proteomic analysis
Inflammatory response
Wear
Graphene
Hyaluronic acid
spellingShingle Química
CoCr
Proteomic analysis
Inflammatory response
Wear
Graphene
Hyaluronic acid
Sánchez López, Luna
Ropero de Torres, Noelia
Chico, Belén
Fagali, Natalia
Ríos, Vivian de los
Escudero,María Lorenza
García Alonso, María Cristina
Lozano, Rosa María
Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages
topic_facet Química
CoCr
Proteomic analysis
Inflammatory response
Wear
Graphene
Hyaluronic acid
description The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications.
format Articulo
Articulo
author Sánchez López, Luna
Ropero de Torres, Noelia
Chico, Belén
Fagali, Natalia
Ríos, Vivian de los
Escudero,María Lorenza
García Alonso, María Cristina
Lozano, Rosa María
author_facet Sánchez López, Luna
Ropero de Torres, Noelia
Chico, Belén
Fagali, Natalia
Ríos, Vivian de los
Escudero,María Lorenza
García Alonso, María Cristina
Lozano, Rosa María
author_sort Sánchez López, Luna
title Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages
title_short Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages
title_full Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages
title_fullStr Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages
title_full_unstemmed Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages
title_sort effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of j774a.1 macrophages
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/152539
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