RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids

Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transf...

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Autores principales: Castellani, Lucas Gabriel, Luchetti, Abril, Nilsson, Juliet Fernanda, Pérez Giménez, Julieta, Struck, Ben, Schlüter, Andreas, Pühler, Alfred, Niehaus, Karsten, Romero, David, Pistorio, Mariano, Torres Tejerizo, Gonzalo Arturo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/151452
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-151452
record_format dspace
spelling I19-R120-10915-1514522023-04-13T20:40:17Z http://sedici.unlp.edu.ar/handle/10915/151452 issn:2150-7511 RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids Castellani, Lucas Gabriel Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Struck, Ben Schlüter, Andreas Pühler, Alfred Niehaus, Karsten Romero, David Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo 2022-09-08 2023-04-13T18:38:47Z en Ciencias Exactas Rhizobia plasmid conjugation Rhizobium gene regulation Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids. Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados". Instituto de Biotecnología y Biología Molecular Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
spellingShingle Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
topic_facet Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
description Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.
format Articulo
Articulo
author Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author_facet Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author_sort Castellani, Lucas Gabriel
title RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_short RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_full RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_fullStr RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_full_unstemmed RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_sort rcga and rcgr, two novel proteins involved in the conjugative transfer of rhizobial plasmids
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/151452
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