The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis
The effect of intraperitoneal administration of α-tocopherol (100 mg/kg wt/24 h) on ascorbate (0.4 mM) induced lipid peroxidation of mitochondria and microsomes isolated from rat liver and testis was studied. Special attention was paid to the changes produced on the highly polyunsaturated fatty acid...
Guardado en:
| Autores principales: | , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2001
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/150740 |
| Aporte de: |
| id |
I19-R120-10915-150740 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias lipid peroxidation α-tocopherol mitochondria microsomes liver testis |
| spellingShingle |
Ciencias Veterinarias lipid peroxidation α-tocopherol mitochondria microsomes liver testis Gavazza, Mariana Beatriz Catalá, Ángel The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| topic_facet |
Ciencias Veterinarias lipid peroxidation α-tocopherol mitochondria microsomes liver testis |
| description |
The effect of intraperitoneal administration of α-tocopherol (100 mg/kg wt/24 h) on ascorbate (0.4 mM) induced lipid peroxidation of mitochondria and microsomes isolated from rat liver and testis was studied. Special attention was paid to the changes produced on the highly polyunsaturated fatty acids C20:4 n6 and C22:6 n3 in liver and C20:4 n6 and C22:5 n6 in testis. The lipid peroxidation of liver mitochondria or microsomes produced a significant decrease of C20:4 n6 and C22:6 n3 in the control group, whereas changes in the fatty acid composition of the α-tocopherol treated group were not observed. The light emission was significantly higher in the control than in the α-tocopherol treated group. The lipid peroxidation of testis microsomes isolated from the α-tocopherol group produced a significant decrease of C20:4 n6 , C22:5 n6 and C22:6 n3, these changes were not observed in testis mitochondria. The light emission of both groups was similar. The treatment with α-tocopherol at the dose and times indicated showed a protector effect on the polyunsaturated fatty acids of liver mitochondria, microsomes and testis mitochondria, whereas those fatty acids situated in testis microsomes were not protected during non enzymatic ascorbate- Fe2+ lipid peroxidation. The protector effect observed by α-tocopherol treatment in the fatty acid composition of rat testis mitochondria but not in microsomes could be explained if we consider that the sum of C20:4 n6 + C22:5 n6 in testis microsomes is 2-fold than that present in mitochondria. |
| format |
Articulo Articulo |
| author |
Gavazza, Mariana Beatriz Catalá, Ángel |
| author_facet |
Gavazza, Mariana Beatriz Catalá, Ángel |
| author_sort |
Gavazza, Mariana Beatriz |
| title |
The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| title_short |
The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| title_full |
The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| title_fullStr |
The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| title_full_unstemmed |
The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| title_sort |
effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis |
| publishDate |
2001 |
| url |
http://sedici.unlp.edu.ar/handle/10915/150740 |
| work_keys_str_mv |
AT gavazzamarianabeatriz theeffectofatocopherolonthelipidperoxidationofmitochondriaandmicrosomesobtainedfromratliverandtestis AT catalaangel theeffectofatocopherolonthelipidperoxidationofmitochondriaandmicrosomesobtainedfromratliverandtestis AT gavazzamarianabeatriz effectofatocopherolonthelipidperoxidationofmitochondriaandmicrosomesobtainedfromratliverandtestis AT catalaangel effectofatocopherolonthelipidperoxidationofmitochondriaandmicrosomesobtainedfromratliverandtestis |
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Repositorios |
| _version_ |
1764820462057029635 |