Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina

Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1,...

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Autores principales: De Felice, Lorena Alejandra, Moré, Gastón Andrés, Cappuccio, Javier Alejandro, Venturini, María Cecilia, Unzaga, Juan Manuel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/150309
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-150309
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
spellingShingle Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
De Felice, Lorena Alejandra
Moré, Gastón Andrés
Cappuccio, Javier Alejandro
Venturini, María Cecilia
Unzaga, Juan Manuel
Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
topic_facet Ciencias Veterinarias
Cryptosporidium suis
Cryptosporidium scrofarum
Piglets
Oocysts
Microscopy
Nested PCR
description Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.
format Articulo
Articulo
author De Felice, Lorena Alejandra
Moré, Gastón Andrés
Cappuccio, Javier Alejandro
Venturini, María Cecilia
Unzaga, Juan Manuel
author_facet De Felice, Lorena Alejandra
Moré, Gastón Andrés
Cappuccio, Javier Alejandro
Venturini, María Cecilia
Unzaga, Juan Manuel
author_sort De Felice, Lorena Alejandra
title Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_short Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_full Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_fullStr Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_full_unstemmed Molecular characterization of <i>Cryptosporidium</i> spp. from domestic pigs in Argentina
title_sort molecular characterization of <i>cryptosporidium</i> spp. from domestic pigs in argentina
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/150309
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