Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides

The aim of this study was evaluate the survival ability of canine and feline spermatozoa maintained within epididymides stored at 4 8C for 24, 48 or 72 h in sterile isotonic saline solution (SAL) or a Tris–egg yolk (TEY) storage medium. Fifteen domestic dogs and 15 cats were neutered and their teste...

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Detalles Bibliográficos
Autores principales: Tittarelli, Claudia Marcela, Savignone, César Augusto, Arnaudín, Evangelina, Stornelli, María Cecilia, Stornelli, María Alejandra, Sota, Rodolfo Luzbel de la
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Veterinarias
Epididymides
Sperm
Storage media
Dog
Cat
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/149285
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-149285
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Epididymides
Sperm
Storage media
Dog
Cat
spellingShingle Ciencias Veterinarias
Epididymides
Sperm
Storage media
Dog
Cat
Tittarelli, Claudia Marcela
Savignone, César Augusto
Arnaudín, Evangelina
Stornelli, María Cecilia
Stornelli, María Alejandra
Sota, Rodolfo Luzbel de la
Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
topic_facet Ciencias Veterinarias
Epididymides
Sperm
Storage media
Dog
Cat
description The aim of this study was evaluate the survival ability of canine and feline spermatozoa maintained within epididymides stored at 4 8C for 24, 48 or 72 h in sterile isotonic saline solution (SAL) or a Tris–egg yolk (TEY) storage medium. Fifteen domestic dogs and 15 cats were neutered and their testes were placed in TEYor SAL and stored at 4 8C for either 24, 48 or 72 h. Sperm samples were obtained by cutting the cauda epididymides into a Tris extender and were evaluated for motility, velocity, viability, plasma membrane integrity, and acrosome morphology. In dogs, there were no significant differences between storage media for motility, plasma membrane integrity, viability and velocity. However, dog sperm stored in TEY had better acrosome morphology compared to sperm stored in SAL (P < 0.05). Dog sperm recovered at 72 h had a reduction in all parameters studied compared to those recovered at 24 h (P < 0.05). In cats, sperm recovered from epididymides stored in TEY had higher motility, plasma membrane integrity and velocity at all times compared to those stored in SAL (P < 0.05). Cat sperm recovered at 72 h had reduced motility, acrosome morphology, viability and velocity compared to those recovered at 24 h (P < 0.05). The addition of TEY to canine epididymal sperm, thus, had a better protective effect than SAL only on acrosome morphology. In cats, in contrast, TEY had a better protective effect than SAL on all epididymal sperm parameters studied. In both species, sperm recovered at 72 h had a significant reduction in all parameters studied compared to those recovered at 24 h.
format Articulo
Articulo
author Tittarelli, Claudia Marcela
Savignone, César Augusto
Arnaudín, Evangelina
Stornelli, María Cecilia
Stornelli, María Alejandra
Sota, Rodolfo Luzbel de la
author_facet Tittarelli, Claudia Marcela
Savignone, César Augusto
Arnaudín, Evangelina
Stornelli, María Cecilia
Stornelli, María Alejandra
Sota, Rodolfo Luzbel de la
author_sort Tittarelli, Claudia Marcela
title Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
title_short Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
title_full Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
title_fullStr Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
title_full_unstemmed Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
title_sort effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/149285
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